After getting an alert, the radar scientists zoned in on the asteroid, which was coming from Earth’s blind spot — solar opposition. Zambrano-Marin and the team had 30 minutes to get as many radar readings as they could. It was traveling so fast, that’s all the time she’d have it in Arecibo’s sights. UCF manages the Arecibo Observatory for the U.S. National Science Foundation under a cooperative agreement.

The asteroid made headline news because it appeared to come out of nowhere and was traveling fast.

Zambrano-Marin’s findings were published in the Planetary Science Journal June 10, just a few weeks before the world observes Asteroid Day, which is June 30 and promotes global awareness to help educate the public about these potential threats.

“It was a real challenge,” says Zambrano-Marin, a UCF planetary scientist. “No one saw it until it was practically passing by, so when we got the alert, we had very little time to act. Even so, we were able to capture a lot of valuable information.”

Turns out the asteroid was between .04 and .08 miles in diameter and was moving fast. It was rotating at 3 to 5 minutes. That means it is part of only 4.2 percent of the known fast rotating asteroids. This is a growing group that the researchers say need more attention.

The data indicates that the asteroid is likely a C-type, which are made up of clay and silicate rocks, or S-type, which are made up of silicate and nickel-iron. C-type asteroids are among the most common and some of the oldest in our solar system. S-type are the second most common.

Zambrano-Marin is now inspecting the data collected through Arecibo’s Planetary Radar database to continue her research. Although the observatory’s telescope collapsed in 2020, the Planetary Radar team can tap the existing data bank that spans four decades. Science operations continue in the areas of space and atmospheric sciences, and the staff is refurbishing 12-meter antennae to continue with astronomy research.

“We can use new data from other observatories and compare it to the observations we have made here over the past 40 years,” Zambrano-Marin says. “The radar data not only helps confirm information from optical observations, but it can help us identify physical and dynamical characteristics, which in turn could give us insights into appropriate deflection techniques if they were needed to protect the planet.”

There are almost 30,000 known asteroids according to Center for Near Earth Studies and while few pose an immediate threat, there is a chance one of significant size could hit the earth and cause catastrophic damage. That’s why NASA keeps a close watch and system to detect and characterize objects once they are found. NASA and other space agencies nations have been launching missions to explore Near-Earth Asteroids to better understand what they are made of and how they move in anticipation of having to divert one heading for earth in the future.

The OSIRIS REx mission, which includes UCF Pegasus Professor of Physics Humberto Campins, is headed back to Earth with a sample of asteroid Bennu, which gave scientists a few surprises. Bennu was first observed at Arecibo in 1999. A new mission — NASA’s Double Asteroid Redirection Test (DART) mission — aims to demonstrate the ability to redirect an asteroid using the kinetic energy of a projectile. The spacecraft launched in November 2021 and is expected to reach its target — the Dimorphos asteroid — on September 26, 2022.

Zambrano-Marin and the rest of the team at Arecibo are working on providing the scientific community with more information about the many kinds of asteroids in the solar system to help come up with contingency plans.

This week the team at the Arecibo Observatory is holding a series of special events as part of the Asteroid Day awareness campaign. They include presentations, “ask a scientist” stations for those visiting the science museum at Arecibo, and on June 25 presentations about the DART mission in English and Spanish. The timing couldn’t be better as there are five known asteroids from the size of a car to a Boeing 747 that will be buzzing Earth before Asteroid Day, according to the Jet Propulsion Laboratory that keeps track of the celestial bodies for NASA. The closest approach is on June 25 with an object coming within 475,000 miles of Earth. For comparison, the moon is about 239,000 miles from Earth.

Zambrano-Marin has multiple degrees including a bachelor’s degree in applied physics from the Ana G. Mendez University System and a master’s in space sciences from the International Space University in France. She has published more than 20 articles and is a frequent speaker and presenter at conference around the world. She previously worked at the Vatican Observatory and as a consultant to the Caribbean University president. In addition to working on the planetary radar group at Arecibo, Zambrano-Marin also created the Arecibo Observatory Space Academy, an 18-week research program for pre-college students in Puerto Rico.

The other team members on the study are: Sean Marshal, Maxime Devogele, Anne Virkki, and Flaviane Venditti from the Arecibo Observatory/UCF; Dylan C. Hickson formerly from Arecibo/UCF and now at Center for Wave Phenomena, Colorado School of Mines; Ellen S. Howell from Lunar and Planetary Laboratory, University of Arizona, Tucson; Patrick Taylor and Edgard Rivera-Valentin from Lunar and Planetary Institute, Universities Space Research Association, Houston; and Jon Giorgini from Solar System Dynamics Group, Jet Propulsion Laboratory.

In this new report, The Ohio State University Comprehensive Cancer Center — Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC — James) researchers identify and describe key features of a gene mutation responsible for 15 to 20% of all melanomas.

Using a preclinical laboratory model, the team establishes that the frequency at which a specific NRAS gene mutation occurs in human melanoma is directly related to the ability of that gene mutation to initiate spontaneous melanoma formation.

“This means that properties of the mutant itself — rather than the ease at which that specific gene mutation occurs — is the cause of cancer formation,” said corresponding author Christin Burd who serves an associate professor of molecular genetics in The Ohio State University College of Arts and Sciences, Department of Molecular Genetics and a member of the OSUCCC — James Molecular Carcinogenesis and Chemoprevention Program.

“NRAS-mutant cancers are challenging to treat because effective therapies beyond immunotherapy don’t exist yet,” said Burd. “Each cancer type seems to prefer a specific ‘flavor’ of mutant NRAS, and it has not been clear why this is.”

The OSUCCC — James wanted to know what made the melanoma-promoting NRAS mutants different than those that promote other cancer types. Scientists say this knowledge could help investigators pinpoint the early events required for melanoma formation and develop treatments that prevent the disease.

Burd and colleagues report their findings in the June 7, 2022, issue of Nature Communications.

Study design and methods

To conduct this study, OSUCCC — James researchers developed genetically engineered models that would allow them to activate one of nine different NRAS-mutant variations in melanocytes, the pigment cells that form melanoma.

“Amazingly, when we activated these gene mutations only those found in the human disease caused melanoma to develop,” said Burd. “Some mutants never led to melanoma, yet we know that they cause leukemia. This finding shows that selection of NRAS mutations is specific to each tumor type and occurs during cancer initiation, rather than in response to a specific mutagenic event like sun exposure.”

Collaborating with Sharon Campbell, a structural biologist at the University of North Carolina (UNC) Chapel Hill, and Debbie Morrison at the National Institutes of Health, Burd’s team identified that slight variances in the outward facing structure of NRAS mutants capable of initiating melanoma that made these proteins better able to interact with the signaling pathways that drive melanoma growth.

“Now we will work to target this unique structural feature of the melanoma-inducing NRAS mutants to prevent and/or treat the disease,” said Burd. “Our work also demonstrates and confirms what was — until now — only speculation: that minor differences between the RAS mutants determine which ‘flavors’ can cause a particular cancer. Such a concept could be used to find vulnerabilities in other RAS-driven tumor types.”

To facilitate similar discoveries, the team generated eight new and publicly available genetically engineered mouse models that will serve as an essential toolkit for the entire RAS community. Burd says these models can be used to activate and study the role of NRAS is other relevant cancer types such as colon cancer, leukemia, myeloma, and thyroid cancer. They can also be used to investigate new drugs for these diseases.

This research was supported with funding from the Damon Runyon Foundation and the Pelotonia Graduate Fellowship of study coauthor Brandon Murphy, who is now a postdoctoral fellow at the University of Utah.

The preclinical research models developed in Burd’s lab and used for this research are publicly available to scientists conducting research about other RAS-driven cancer types or working to develop new cancer therapies through the National Institute of Health’s repository.

Additional study coauthors include Elizabeth M. Terrell, Venkat R. Chirasani, Tirzah J. Weiss, Rachel E. Lew, Andrea M. Holderbaum, Aastha Dhakal, Valentina Posada, Marie Fort, Michael S. Bodnar, Leiah M. Carey, Min Chen, Craig J. Burd, Vincenzo Coppola and Sharon L. Campbell.

Megatooth sharks get their name from their massive teeth, which can each be bigger than a human hand. The group includes Megalodon, the largest shark that ever lived, as well as several related species.

While sharks of one kind or another have existed since long before the dinosaurs — for more than 400 million years — these megatooth sharks evolved after the dinosaurs went extinct and ruled the seas until just 3 million years ago.

“We’re used to thinking of the largest species — blue whales, whale sharks, even elephants and diplodocuses — as filter feeders or herbivores, not predators,” said Emma Kast, a 2019 Ph.D. graduate in geosciences who is the first author on a new study in the current issue of Science Advances. “But Megalodon and the other megatooth sharks were genuinely enormous carnivores that ate other predators, and Meg went extinct only a few million years ago.”

Her adviser Danny Sigman, Princeton’s Dusenbury Professor of Geological and Geophysical Sciences, added, “If Megalodon existed in the modern ocean, it would thoroughly change humans’ interaction with the marine environment.”

A team of Princeton researchers has now discovered clear evidence that Megalodon and some of its ancestors were at the very highest rung of the prehistoric food chain — what scientists call the highest “trophic level.” Indeed, their trophic signature is so high that they must have eaten other predators and predators-of-predators in a complicated food web, say the researchers.

“Ocean food webs do tend to be longer than the grass-deer-wolf food chain of land animals, because you start with such small organisms,” said Kast, now at the University of Cambridge, who wrote the first iteration of this research as a chapter in her dissertation. “To reach the trophic levels we’re measuring in these megatooth sharks, we don’t just need to add one trophic level — one apex predator on top of the marine food chain — we need to add several onto the top the modern marine food web.”

Megalodon has been conservatively estimated at 15 meters long — 50 feet — while modern great white sharks typically top out around five meters (15 feet).

To reach their conclusions about the prehistoric marine food web, Kast, Sigman and their colleagues used a novel technique to measure the nitrogen isotopes in the sharks’ teeth. Ecologists have long known that the more nitrogen-15 an organism has, the higher its trophic level, but scientists have never before been able to measure the tiny amounts of nitrogen preserved in the enamel layer of these extinct predators’ teeth.

“We have a series of shark teeth from different time periods, and we were able to trace their trophic level versus their size,” said Zixuan (Crystal) Rao, a graduate student in Sigman’s research group and a co-author on the current paper.

One way to tuck in an extra trophic level or two is cannibalism, and several lines of evidence point to that in both megatooth sharks and other prehistoric marine predators.

The nitrogen time machine

Without a time machine, there’s no easy way to recreate the food webs of extinct creatures; very few bones have survived with teeth marks that say, “I was chewed on by a massive shark.”

Fortunately, Sigman and his team have spent decades developing other methods, based on the knowledge that the nitrogen isotope levels in a creature’s cells reveal whether it is at the top, middle or bottom of a food chain.

“The whole direction of my research team is to look for chemically fresh, but physically protected, organic matter — including nitrogen — in organisms from the distant geologic past,” said Sigman.

A few plants, algae and other species at the bottom of the food web have mastered the knack of turning nitrogen from the air or water into nitrogen in their tissues. Organisms that eat them then incorporate that nitrogen into their own bodies, and critically, they preferentially excrete (sometimes via urine) more of nitrogen’s lighter isotope, N-14, than its heavier cousin, N-15.

In other words, N-15 builds up, relative to N-14, as you climb up the food chain.

Other researchers have used this approach on creatures from the recent past — the most recent 10-15 thousand years — but there hasn’t been enough nitrogen left in older animals to measure, until now.

Why? Soft tissue like muscles and skin are hardly ever preserved. To complicate matters, sharks don’t have bones — their skeletons are made of cartilage.

But sharks do have one golden ticket into the fossil record: teeth. Teeth are more easily preserved than bones because they are encased in enamel, a rock-hard material that is virtually immune to most decomposing bacteria.

“Teeth are designed to be chemically and physically resistant so they can survive in the very chemically reactive environment of the mouth and break apart food that can have hard parts,” Sigman explained. And in addition, sharks aren’t limited to the 30 or so pearly whites that humans have. They are constantly growing and losing teeth — modern sand sharks lose a tooth every day of their decades-long lives, on average — which means that every shark produces thousands of teeth over its lifetime.

“When you look in the geologic record, one of the most abundant fossil types are shark teeth,” said Sigman. “And within the teeth, there is a tiny amount of organic matter that was used to build the enamel of the teeth — and is now trapped within that enamel.”

Since shark teeth are so abundant and are preserved so well, the nitrogen signatures in enamel provide a way to measure status in the food web, whether the tooth fell from a shark’s mouth millions of years ago or yesterday.

Even the largest tooth has only a thin casing of enamel, of which the nitrogen component is only a tiny trace. But Sigman’s team has been developing more and more refined techniques for extracting and measuring these nitrogen isotope ratios, and with a little help from dentist drills, cleaning chemicals and microbes that ultimately convert the nitrogen from within the enamel into nitrous oxide, they’re now able to precisely measure the N15-N14 ratio in these ancient teeth.

“We’re a little bit like a brewery,” he said. “We grow microbes and feed our samples to them. They produce nitrous oxide for us, and then we analyze the nitrous oxide they produced.”

The analysis requires a custom-built, automated nitrous oxide preparation system that extracts, purifies, concentrates and delivers the gas to a specialized stable isotope ratio mass spectrometer.

“This has been a multiple-decades-long quest that I’ve been on, to develop a core method to measure these trace amounts of nitrogen,” Sigman said. From microfossils in sediments, they moved on to other types of fossils, like corals, fish ear bones and shark teeth. “Next, we and our collaborators are applying this to mammalian teeth and dinosaur teeth.”

A deep dive into the literature during lockdown

Early in the pandemic, while her friends were making sourdough starters and bingeing Netflix, Kast pored through the ecologic literature to look for nitrogen isotope measurements of modern marine animals.

“One of the cool things that Emma did was really dig into the literature — all the data that’s been published over decades — and relate that to the fossil record,” said Michael (Mick) Griffiths, a paleoclimatologist and geochemist at William Patterson University and a co-author on the paper.

As Kast quarantined at home, she painstakingly built up a record with more than 20,000 marine mammal individuals and more than 5,000 sharks. She wants to take things much further. “Our tool has the potential to decode ancient food webs; what we need now is samples,” said Kast. “I’d love to find a museum or other archive with a snapshot of an ecosystem — a collection of different kinds of fossils from one time and place, from forams near the very base of the food web, to otoliths — inner ear bones — from different kinds of fish, to teeth from marine mammals, plus shark teeth. We could do the same nitrogen isotope analysis and put together the whole story of an ancient ecosystem.”

In addition to the literature search, their database includes their own samples of shark teeth. Co-author Kenshu Shimada of DePaul University connected with aquariums and museums, while co-authors Martin Becker of William Patterson University and Harry Maisch of Florida Gulf Coast University gathered megatooth specimens on the sea floor.

“It’s really dangerous; Harry’s a dive master, and you really need to be an expert to get these,” said Griffiths. “You can find little shark teeth on the beach, but to get the best-preserved samples, you need to go down to the bottom of the ocean. Marty and Harry have collected teeth from all over the place.”

Without clear road signs to indicate where native islanders were traveling, however, the task of reconstructing ancient trade routes relies on subtle clues locked away in the archaeological record. Researchers at the Florida Museum of Natural History recently turned to pottery to tease apart the navigational history of the Caribbean, analyzing the composition of 96 fired clay fragments across 11 islands.

The study, published in the Journal of Archaeological Science: Reports, is the broadest of its kind yet conducted in the Greater Antilles and marks the first time that pottery artifacts from the Lucayan Islands — The Bahamas plus the Turks and Caicos Islands — have been analyzed to determine their elemental composition and origin.

“Our methods mark a big improvement over other studies that mostly look at a single site or single island, where you might see differences but not know what it means because you’re looking at the results in isolation,” said co-author Lindsay Bloch, a courtesy faculty member with the Florida Museum’s Ceramic Technology Lab.

People have lived on the Caribbean islands on and off for more than 7,000 years, migrating in waves from Central and South America. As early as 800 B.C., new groups arrived from Venezuela and established a trading network among islands, which they used to exchange food, tools and jewelry. But the most common artifacts that survived to the present are the pottery vessels these objects were carried in.

“Most materials don’t preserve well in the Caribbean because of the warm, humid environment, but pottery is durable, so it ends up being one of the most common things we find,” said lead author Emily Kracht, a collections assistant in the Ceramic Technology Lab.

Over the ensuing millennia, different Caribbean cultures developed unique styles and techniques for constructing their pottery. Some artifacts are simple and unadorned, while others are highly decorated, with a lattice of incised lines, punctations, raised ridges and flared rims.

Many studies have relied almost entirely on similarities in style to distinguish between different cultures and infer their movements. But, as Bloch explains, this method has often left more questions than answers and excludes material with potentially valuable information.

“The vast majority of pottery that we find anywhere in the world is going to be undecorated. It’s going to be things used for cooking or storage, which are typically plain and often get ignored because they’re seen as generic,” she said.

Rather than studying the minutiae of varying styles, the researchers focused instead on what the pottery was made of. Using a laser to etch microscopic lines into their samples, the researchers determined the exact amounts and identities of each element in the clay used to make the pottery. Their final analysis included more than seven decades’ worth of archaeological collections that span over 1,000 years of Indigenous Caribbean history.

“One of the advantages of elemental analysis is that we’re explicitly looking for differences, which allows us to see where a pot was made and compare that to where it ended up,” Bloch said.

Such detailed comparisons are possible due to the complexity of the Caribbean’s underlying geology. The largest islands in the archipelago likely got their start as an ancient underwater plateau in the Pacific Ocean. After the breakup of the supercontinent Pangaea, the Caribbean plate drifted east in a flurry of volcanic eruptions that elevated the plateau above sea level before ultimately reaching its current position in the Atlantic.

Millions of years of weathering reduced these volcanic outcrops into fine-grained clays with differing concentrations of elements like copper, nickel, chromium and antimony. These differences mean that even the smallest Caribbean pottery sherd bears the elemental signature of the region it was made in.

The results of researchers’ comparative analysis aren’t what might be expected by simply looking at a map. The Lucayan Islands were initially used only temporarily for harvesting resources, and the people who traveled to them would have set sail from the larger islands to the south that supported permanent population centers.

Cuba might initially seem like it’s the perfect staging ground for these operations, being by far the largest Caribbean island and the closest to The Bahamas. While people did make the trek across open water from Cuba, the results of the study indicate the Caribbean’s cultural hub was instead centered on the northwest coast of Hispaniola, from which people imported and exported goods for hundreds of years.

“At least some of the pottery would have been used to ferry goods out to these islands, and people would potentially carry back a variety of marine resources,” Bloch said.

People eventually struck up permanent settlements in The Bahamas and Turks and Caicos, becoming collectively known as the Lucayans, or the People of the Islands. They began making their own pottery from claylike soils deposited by African dust plumes blown in from the Saharan Desert, but the results didn’t quite hold up to the pottery from Hispaniola — literally. Lucayan pottery, called Palmetto Ware, is most often thick and soft and crumbles over time due to the poor quality of the grainy Saharan soil.

Thus, up until the arrival of the Spanish, Hispaniola remained the main trading partner and exporter of pottery to the Lucayan Islands.

“We knew that the Lucayans were related to people in Hispaniola, and this study shows their enduring relationship over hundreds of years through pottery,” Kracht said.

William Keegan of the Florida Museum of Natural History is also a co-author on the study.