Scientists from the University of Cambridge studied hundreds of tree trunks, dug up by Fenland farmers while ploughing their fields. The team found that most of the ancient wood came from yew trees that populated the area between four and five thousand years ago.

These trees, which are a nuisance when they jam farming equipment during ploughing, contain a treasure trove of perfectly preserved information about what the Fens looked like thousands of years ago.

The Fen yew woodlands suddenly died about 4,200 years ago, when the trees fell into peat and were preserved until today. The researchers hypothesise that a rapid sea level rise in the North Sea flooded the area with salt water, causing the vast woodlands to disappear.

The climate and environmental information these trees contain could be a valuable clue in determining whether this climate event could be related to other events that happened elsewhere in the world at the same time, including a megadrought in the Middle East that may have been a factor in the collapse of ancient Egypt’s Old Kingdom. Their results are reported in the journal Quaternary Science Reviews.

Yew (Taxus baccata) trees are one of the longest-lived species in Europe, and can reach up to 20 metres in height. While these trees are fairly common in Cambridge College gardens and churchyards across southern England, they are absent in the Fens, the low-lying marshy region of eastern England. Much of the Fens was a wetland until it was drained between the 17^th and 19^th centuries using artificial drainage and flood protection. Today, the area is some of the most productive farmland in the UK, thanks to its rich peat soil.

While the area is great for farming and does have its own charms, few people would describe the Fens as spectacular: for the most part, the area is extremely flat and dominated by fields of potatoes, sugar beet, wheat and other crops. But five thousand years ago, the area was a huge forest.

“A common annoyance for Fenland farmers is getting their equipment caught on big pieces of wood buried in the soil, which can often happen when planting potatoes, since they are planted a little deeper than other crops,” said lead author Tatiana Bebchuk, a PhD student from Cambridge’s Department of Geography. “This wood is often pulled up and piled at the edge of fields: it’s a pretty common sight to see these huge piles of logs when driving through the area.”

For farmers, these logs are a nuisance. But for Bebchuk and her colleagues, they are buried treasure. The Cambridge team approached several Fenland farmers and took samples of hundreds of logs that had been dug up and discarded, to find out what secrets they might hold.

“I remember when I first saw this enormous pile of abandoned trees, it was incredible just how many there were,” said Bebchuk. “But when we got them back to lab, we were even more surprised: these trees were so well-preserved, it looked as if they were cut down just yesterday.”

To put current anthropogenic climate change in a long-term context of natural variability, scientists need accurate evidence from the past, and trees are some of the best recorders of past conditions: their annual growth rings contain information about temperature and hydroclimate for every growing season they witnessed. “But the further back in time we go, the less reliable evidence we have, since very old trees and well-preserved wood materials are extremely rare,” said Professor Ulf Büntgen, the senior author of the study.

However, analysis by the Cambridge Tree-Ring Unit (TRU) showed that the yew trees dug up from Fenland fields were very old indeed: some of these ancient trees were 400 years old when they died. The new find provides unique climate information for over a millennium from around 5,200 years ago until about 4,200 years ago, when much of the Fens was a woodland of yew and oak: completely different than it looks today.

“Finding these very old trees in the Fens is completely unexpected — it would be like turning a corner in rural Cambridgeshire and seeing an Egyptian pyramid — you just wouldn’t expect it,” said Bebchuk. “It’s the same with nature — wood rots and decomposes easily, so you just don’t expect a tree that died five or four thousand years ago to last so long.”

Given that most of the Fens are barely above sea level, about 4,200 years ago, a sudden rise in sea level most likely killed the Fen woodlands. The period that the Fen woodlands died coincided with major climatic changes elsewhere in the world: at roughly the same time, a megadrought in China and the Middle East was a possible trigger of the collapse of several civilisations, including Egypt’s Old Kingdom and the Akkadian Empire in Mesopotamia.

“We want to know if there is any link between these climatic events,” said Bebchuk. “Are the megadroughts in Asia and the Middle East possibly related to the rapid sea level rise in northern Europe? Was this a global climate event, or was it a series of unrelated regional changes? We don’t yet know what could have caused these climate events, but these trees could be an important part of solving this detective story.”

“This is such a unique climate and environmental archive that will provide lots of opportunities for future studies, and it’s right from Cambridge’s own backyard,” said Büntgen. “We often travel all over the world to collect ice cores or ancient trees, but it’s really special to find such a unique archive so close to the office.”

Our bodies are, in some ways, like cars — to keep functioning, they need to be checked and repaired regularly. In the case of our bodies, any cells that are damaged or dead need to be replaced to keep organs functioning. This replacement occurs thanks to tissue-resident adult stem cells. In contrast with embryonic stem cells, which can form any cell type in the body, adult stem cells will only form the cell types that are found in the tissue they belong to. But how do tissue-specific stem cells know which cell type to give rise to? Gabriele Colozza, a postdoctoral researcher in the lab of Bon-Kyoung Koo at IMBA — now director at the Center for Genome Engineering, Institute for Basic Science in South Korea — decided to investigate this question using intestinal stem cells.

Intestines — a constant construction site

“In our intestines, cells are exposed to extreme conditions,” Colozza explains. Mechanical wear and tear, but also digestive enzymes and varying pH values all affect intestinal cells. In turn, stem cells in the intestine’s mucosa differentiate to form new intestinal cells. “Damaged cells have to be replaced, but it is a delicate balance between stem cell renewal and differentiation into other cell types: uncontrolled stem cell proliferation may lead to tumor formation; on the other hand, if too many stem cells differentiate, the tissue will be depleted of stem cells and ultimately unable to self-renew.”

This balance is delicately tuned by signaling pathways and feedback loops, which allow cells to communicate with each other. One important pathway is called Wnt. The Wnt pathway is known for its role in embryonic development, and if left unchecked, an overactive Wnt pathway can lead to excessive cell division and the formation of tumors.

Molecular partner identified

A well-known antagonist of Wnt signalling — keeping Wnt in check — is Rnf43, which was originally identified by Bon-Kyoung Koo. Prior to this study, Rnf43 was known to target the Wnt receptor Frizzled and mark it for degradation. “We wanted to know how Rnf43 works, and also what — in turn — controls Rnf43 and helps it to regulate Wnt signalling.” From earlier research, the scientists knew that Rnf43 on its own was not sufficient to break down the Wnt receptor Frizzled, which sits in the plasma membrane. “In our project, we used biochemical assays to identify which proteins interact with Rnf43.” A key partner of Rnf43 turned out to be the protein Daam1.

To understand how Daam1 regulates Rnf43 and affects the tissues it acts in, Colozza turned to intestinal organoids. “We found that Daam1 is required for Rnf43 to be active, so for Rnf43 to regulate Wnt signaling at all. Further work in cells showed Rnf43 needs Daam1 to move the Wnt receptor Frizzled into vesicles called endosomes. From the endosomes, Frizzled is shuttled to the lysosomes where it is degraded, dampening Wnt signaling,” Colozza adds.

Intestinal organoids are three-dimensional cell cultures grown from adult intestinal stem cells, allowing the researchers to mimic the intestinal mucosa. For Colozza, organoids were an opportunity to understand how Rnf43 and Daam1 affect the delicate balance of stem cell renewal and differentiation in the intestine. “We found that when we knock-out Rnf43 or Daam1, the organoids grow into tumor-like structures. These tumor-like organoids keep on growing, even if we withdraw the growth factors they usually depend on, such as R-spondin.”

Switching on Paneth cell formation

When Colozza followed up this result in mouse tissue, the researchers were in for a surprise. “When Rnf43 was missing, the intestines grew tumors — as expected. But when Daam1 was missing, no tumors grew. We were puzzled by this striking difference: how can the loss of factors in the same pathway, that behave similarly in organoids, lead to such different outcomes?”

Looking closely at the intestines, Colozza saw that intestines lacking Rnf43 were full of a specific type of secretory cells, the Paneth cells. Intestines lacking Daam1, on the other hand, contained no extra Paneth cells. Paneth cells secrete growth factors, such as Wnt, that stimulate cell division. “Daam1 is required for the efficient formation of Paneth cells. When Daam1 is active, stem cells differentiate to form Paneth cells. When Daam1 is not active, the stem cells differentiate into another cell type.”

Tumors modify their niche to grow

This link between the molecular results and Paneth cells explains the puzzling difference between intestines and organoids. “In organoid culture, we scientists provide growth factors, so the knockout of both Rnf43 and Daam1 lead to tumor-like organoids. But in the intestine, there is no little scientist providing growth factors. Instead, Paneth cells provide growth factors, like Wnt, and create the right conditions for stem cells to survive and divide. When Paneth cells are lacking — such as when Daam1 is not active to drive cells into becoming Paneth cells — stem cells will not divide much. But when there are too many Paneth cells — such as in intestines lacking Rnf43 — the excessive growth factors can contribute to the formation of tumors.”

Colozza’s and colleagues’ study is the first genetic proof that Daam1, a member of the non-canonical Wnt pathway, is important for specifying Paneth cells, and directly involved in the development of this crucial secretory cell. The results also shed light on the importance of the stem cell niche. “We show that tumor cells modify their microenvironment, and influence their supporting environment so that they can grow better.”

“We have only a good record of the deep ocean for the last ~180 million years,” said David Fike, the Glassberg/Greensfelder Distinguished University Professor of Earth, Environmental, and Planetary Sciences in Arts & Sciences at Washington University in St. Louis. “Everything else is just shallow-water deposits. So it’s really important to understand the bias that might be present when we look at shallow-water deposits.”

One of the ways that scientists like Fike use deposits from the seafloor is to reconstruct timelines of past ecological and environmental change. Researchers are keenly interested in how and when oxygen began to build up in the oceans and atmosphere, making Earth more hospitable to life as we know it.

For decades they have relied on pyrite, the iron-sulfide mineral known as “fool’s gold,” as a sensitive recorder of conditions in the marine environment where it is formed. By measuring the bulk isotopic composition of sulfur in pyrite samples — the relative abundance of sulfur atoms with slightly different mass — scientists have tried to better understand ancient microbial activity and interpret global chemical cycles.

But the outlook for pyrite is not so shiny anymore. In a pair of companion papers published Nov. 24 in the journal Science, Fike and his collaborators show that variations in pyrite sulfur isotopes may not represent the global processes that have made them such popular targets of analysis.

Instead, Fike’s research demonstrates that pyrite responds predominantly to local processes that should not be taken as representative of the whole ocean. A new microanalysis approach developed at Washington University helped the researchers to separate out signals in pyrite that reveal the relative influence of microbes and that of local climate.

For the first study, Fike worked with Roger Bryant, who completed his graduate studies at Washington University, to examine the grain-level distribution of pyrite sulfur isotope compositions in a sample of recent glacial-interglacial sediments. They developed and used a cutting-edge analytical technique with the secondary-ion mass spectrometer (SIMS) in Fike’s laboratory.

“We analyzed every individual pyrite crystal that we could find and got isotopic values for each one,” Fike said. By considering the distribution of results from individual grains, rather than the average (or bulk) results, the scientists showed that it is possible to tease apart the role of the physical properties of the depositional environment, like the sedimentation rate and the porosity of the sediments, from the microbial activity in the seabed.

“We found that even when bulk pyrite sulfur isotopes changed a lot between glacials and interglacials, the minima of our single grain pyrite distributions remained broadly constant,” Bryant said. “This told us that microbial activity did not drive the changes in bulk pyrite sulfur isotopes and refuted one of our major hypotheses.”

“Using this framework, we’re able to go in and look at the separate roles of microbes and sediments in driving the signals,” Fike said. “That to me represents a huge step forward in being able to interpret what is recorded in these signals.”

In the second paper, led by Itay Halevy of the Weizmann Institute of Science and co-authored by Fike and Bryant, the scientists developed and explored a computer model of marine sediments, complete with mathematical representations of the microorganisms that degrade organic matter and turn sulfate into sulfide and the processes that trap that sulfide in pyrite.

“We found that variations in the isotopic composition of pyrite are mostly a function of the depositional environment in which the pyrite formed,” Halevy said. The new model shows that a range of parameters of the sedimentary environment affect the balance between sulfate and sulfide consumption and resupply, and that this balance is the major determinant of the sulfur isotope composition of pyrite.

“The rate of sediment deposition on the seafloor, the proportion of organic matter in that sediment, the proportion of reactive iron particles, the density of packing of the sediment as it settles to the seafloor — all of these properties affect the isotopic composition of pyrite in ways that we can now understand,” he said.

Importantly, none of these properties of the sedimentary environment are strongly linked to the global sulfur cycle, to the oxidation state of the global ocean, or essentially any other property that researchers have traditionally used pyrite sulfur isotopes to reconstruct, the scientists said.

“The really exciting aspect of this new work is that it gives us a predictive model for how we think other pyrite records should behave,” Fike said. “For example, if we can interpret other records — and better understand that they are driven by things like local changes in sedimentation, rather than global parameters about ocean oxygen state or microbial activity — then we can try to use this data to refine our understanding of sea level change in the past.”

“The noise is greatly suppressed compared to ordinary wires,” said Rice’s Douglas Natelson, the study’s corresponding author. “Maybe this is evidence that quasiparticles are not well-defined things or that they’re just not there and charge moves in more complicated ways. We have to find the right vocabulary to talk about how charge can move collectively.”

The experiments were performed on nanoscale wires of a quantum critical material with a precise 1-2-2 ratio of ytterbium, rhodium and silicon (YbRh2Si2), which has been studied in great depth during the past two decades by Silke Paschen, a solid-state physicist at the Vienna University of Technology (TU Wien). The material contains a high degree of quantum entanglement that produces a very unusual (“strange”) temperature-dependent behavior that is very different from the one in normal metals such as silver or gold.

In such normal metals, each quasiparticle, or discrete unit, of charge is the product of incalculable tiny interactions between countless electrons. First put forward 67 years ago, the quasiparticle is a concept physicists use to represent the combined effect of those interactions as a single quantum object for the purposes of quantum mechanical calculations.

Some prior theoretical studies have suggested that the charge in a strange metal might not be carried by such quasiparticles, and shot noise experiments allowed Natelson, study lead author Liyang Chen, a former student in Natelson’s lab, and other Rice and TU Wien co-authors to gather the first direct empirical evidence to test the idea.

“The shot noise measurement is basically a way of seeing how granular the charge is as it goes through something,” Natelson said. “The idea is that if I’m driving a current, it consists of a bunch of discrete charge carriers. Those arrive at an average rate, but sometimes they happen to be closer together in time, and sometimes they’re farther apart.”

Applying the technique in YbRh2Si2 crystals presented significant technical challenges. Shot noise experiments cannot be performed on single macroscopic crystals but, rather, require samples of nanoscopic dimensions. Thus, the growth of extremely thin but nevertheless perfectly crystalline films had to be achieved, something that Paschen, Maxwell Andrews and their collaborators at TU Wien managed after almost a decade of hard work. Next, Chen had to find a way to maintain that level of perfection while fashioning wires from these thin films that were about 5,000 times narrower than a human hair.

Rice co-author Qimiao Si, the lead theorist on the study and the Harry C. and Olga K. Wiess Professor of Physics and Astronomy, said he, Natelson and Paschen first discussed the idea for the experiments while Paschen was a visiting scholar at Rice in 2016. Si said the results are consistent with a theory of quantum criticality he published in 2001 that he has continued to explore in a nearly two-decade collaboration with Paschen.

“The low shot noise brought about fresh new insights into how the charge-current carriers entwine with the other agents of the quantum criticality that underlies the strange metallicity,” said Si, whose group performed calculations that ruled out the quasiparticle picture. “In this theory of quantum criticality, the electrons are pushed to the verge of localization, and the quasiparticles are lost everywhere on the Fermi surface.”

Natelson said the larger question is whether similar behavior might arise in any or all of the dozens of other compounds that exhibit strange metal behavior.

“Sometimes you kind of feel like nature is telling you something,” Natelson said. “This ‘strange metallicity’ shows up in many different physical systems, despite the fact that the microscopic, underlying physics is very different. In copper-oxide superconductors, for example, the microscopic physics is very, very different than in the heavy-fermion system we’re looking at. They all seem to have this linear-in-temperature resistivity that’s characteristic of strange metals, and you have to wonder is there something generic going on that is independent of whatever the microscopic building blocks are inside them.”

The research was supported by the Department of Energy’s Basic Energy Sciences program (DE-FG02-06ER46337), the National Science Foundation (1704264, 2220603), the European Research Council (101055088), the Austrian Science Fund (FWF I4047, FWF SFB F 86), the Austrian Research Promotion Agency (FFG 2156529, FFG 883941), the European Union’s Horizon 2020 program (824109-EMP), the Air Force Office of Scientific Research (FA8665-22-1-7170), the Welch Foundation (C-1411) and the Vannevar Bush Faculty Fellowship (ONR-VB-N00014-23-1-2870).

The Telescope Array has since observed more than 30 ultra-high-energy cosmic rays, though none approaching the Oh-My-God-level energy. No observations have yet revealed their origin or how they are able to travel to the Earth.

On May 27, 2021, the Telescope Array experiment detected the second-highest extreme-energy cosmic ray. At 2.4 x 10²⁰eV, the energy of this single subatomic particle is equivalent to dropping a brick on your toe from waist height. Led by the University of Utah (the U) and the University of Tokyo, the Telescope Array consists of 507 surface detector stations arranged in a square grid that covers 700 km² (~270 miles²) outside of Delta, Utah in the state’s West Desert. The event triggered 23 detectors at the north-west region of the Telescope Array, splashing across 48 km² (18.5 mi²). Its arrival direction appeared to be from the Local Void, an empty area of space bordering the Milky Way galaxy.

“The particles are so high energy, they shouldn’t be affected by galactic and extra-galactic magnetic fields. You should be able to point to where they come from in the sky,” said John Matthews, Telescope Array co-spokesperson at the U and co-author of the study. “But in the case of the Oh-My-God particle and this new particle, you trace its trajectory to its source and there’s nothing high energy enough to have produced it. That’s the mystery of this — what the heck is going on?”

In their observation that published on Nov. 24, 2023, in the journal Science, an international collaboration of researchers describe the ultra-high-energy cosmic ray, evaluate its characteristics, and conclude that the rare phenomena might follow particle physics unknown to science. The researchers named it the Amaterasu particle after the sun goddess in Japanese mythology. The Oh-My-God and the Amaterasu particles were detected using different observation techniques, confirming that while rare, these ultra-high energy events are real.

“These events seem like they’re coming from completely different places in the sky. It’s not like there’s one mysterious source,” said John Belz, professor at the U and co-author of the study. “It could be defects in the structure of spacetime, colliding cosmic strings. I mean, I’m just spit-balling crazy ideas that people are coming up with because there’s not a conventional explanation.”

Natural particle accelerators

Cosmic rays are echoes of violent celestial events that have stripped matter to its subatomic structures and hurled it through universe at nearly the speed of light. Essentially cosmic rays are charged particles with a wide range of energies consisting of positive protons, negative electrons, or entire atomic nuclei that travel through space and rain down onto Earth nearly constantly.

Cosmic rays hit Earth’s upper atmosphere and blasts apart the nucleus of oxygen and nitrogen gas, generating many secondary particles. These travel a short distance in the atmosphere and repeat the process, building a shower of billions of secondary particles that scatter to the surface. The footprint of this secondary shower is massive and requires that detectors cover an area as large as the Telescope Array. The surface detectors utilize a suite of instrumentation that gives researchers information about each cosmic ray; the timing of the signal shows its trajectory and the amount of charged particles hitting each detector reveals the primary particle’s energy.

Because particles have a charge, their flight path resembles a ball in a pinball machine as they zigzag against the electromagnetic fields through the cosmic microwave background. It’s nearly impossible to trace the trajectory of most cosmic rays, which lie on the low- to middle-end of the energy spectrum. Even high-energy cosmic rays are distorted by the microwave background. Particles with Oh-My-God and Amaterasuenergy blast through intergalactic space relatively unbent. Only the most powerful of celestial events can produce them.

“Things that people think of as energetic, like supernova, are nowhere near energetic enough for this. You need huge amounts of energy, really high magnetic fields to confine the particle while it gets accelerated,” said Matthews.

Ultra-high-energy cosmic rays must exceed 5 x 10¹⁹ eV. This means that a single subatomic particle carries the same kinetic energy as a major league pitcher’s fast ball and has tens of millions of times more energy than any human-made particle accelerator can achieve. Astrophysicists calculated this theoretical limit, known as the Greisen-Zatsepin-Kuzmin (GZK) cutoff, as the maximum energy a proton can hold traveling over long distances before the effect of interactions of the microwave background radiation take their energy. Known source candidates, such as active galactic nuclei or black holes with accretion disks emitting particle jets, tend to be more than 160 million light years away from Earth. The new particle’s 2.4 x 10²⁰ eV and the Oh-My-God particle’s 3.2 x 10²⁰ eV easily surpass the cutoff.

Researchers also analyze cosmic ray composition for clues of its origins. A heavier particle, like iron nuclei, are heavier, have more charge and are more susceptible to bending in a magnetic field than a lighter particle made of protons from a hydrogen atom. The new particle is likely a proton. Particle physics dictates that a cosmic ray with energy beyond the GZK cutoff is too powerful for the microwave background to distort its path, but back tracing its trajectory points towards empty space.

“Maybe magnetic fields are stronger than we thought, but that disagrees with other observations that show they’re not strong enough to produce significant curvature at these ten-to-the-twentieth electron volt energies,” said Belz. “It’s a real mystery.”

Expanding the footprint

The Telescope Array is uniquely positioned to detect ultra-high-energy cosmic rays. It sits at about 1,200 m (4,000 ft), the elevation sweet-spot that allows secondary particles maximum development, but before they start to decay. Its location in Utah’s West Desert provides ideal atmospheric conditions in two ways: the dry air is crucial because humidity will absorb the ultraviolet light necessary for detection; and the region’s dark skies are essential, as light pollution will create too much noise and obscure the cosmic rays.

Astrophysicists are still baffled by the mysterious phenomena. The Telescope Array is in the middle of an expansion that that they hope will help crack the case. Once completed, 500 new scintillator detectors will expand the Telescope Array will sample cosmic ray-induced particle showers across 2,900 km² (1,100 mi² ), an area nearly the size of Rhode Island. The larger footprint will hopefully capture more events that will shed light on what’s going on.