Researchers from UNSW Sydney and the Australian Institute of Botanical Science, part of the Royal Botanic Gardens and Domain Trust, surveyed 33 major online databases of plant photographs to examine the photographic record of Australian plant species. The findings, published in New Phytologist, reveal out of 21,077 native Australian vascular plant species, almost 20 per cent lack a verifiable photograph.

Lead author of the study and UNSW Science PhD student Thomas Mesaglio says Australia is one of the richest areas in the world for native species.

“It was surprising to see how many plant species had just line drawings, illustrations, paintings, or even no media at all,” Mr Mesaglio says.

Dr Hervé Sauquet, co-author of the study and Senior Research Scientist at the Australian Institute of Botanical Science, is based at the National Herbarium of New South Wales.

“All species of plants ultimately rely on specimens in herbarium collections for their identification,” Dr Sauquet says. “Yet, even in this digital age where most herbarium specimens have been scanned and are accessible on the web, photos of live plants in the wild remain in critical need.”

Senior author of the study from UNSW Science Associate Professor Will Cornwell says a lack of detailed photos can have real consequences. Many plant species that are difficult to identify in the wild may go extinct if scientists cannot properly identify them with the help of photos.

“We had assumed every plant species would have simply been photographed by someone, somewhere, throughout history. But it turns out this isn’t the case,” says A/Prof. Cornwell.

“This is where citizen scientists can come in and help us fill this gap with their photos.”

Gaps in the photographic record

Photographs can help botanists and taxonomists who work with plant specimens by preserving characteristics like flower colour that get lost over time in their samples. They can also show additional features, such as the orientation of leaves or bark appearance, and add ecological context.

“Having a comprehensive photographic set helps us to be confident in our identifications,” Mr Mesaglio says. “Particularly when it is practically challenging to collect and preserve the entire plant, photos complement the physical voucher by showing the soil type, the habitat it’s growing in, and other species growing alongside it.”

But it turns out not all plant groups are photographed equally. Just as some animals receive less attention than others, there might also be a bias against less charismatic plants.

The study found the most well-photographed plant groups tend to be shrubs or trees with more noticeable or spectacular features, such as colourful flowers. Banksia, for example, is one of only two Australian plant genera with more than 40 species to have a complete photographic record. Meanwhile, the family with the most significant photo deficit was Poaceae — commonly known as grasses — with 343 unphotographed species.

“We noticed a charisma deficit, so the species that tend to be harder to see are the ones missing out,” Mr Mesaglio says. “They may have innocuous or pale-looking flowers or be smaller and harder to spot grasses, sedges and herbs.”

Geography also affected the photographic record. While most species across the south-eastern states of Australia have comprehensive records, Western Australia had the largest void, with 52 per cent of all unphotographed species found there.

“The primary ‘hotspots’ for unphotographed Australian plants are areas with high plant diversity, but the environments are rugged and often difficult to access, particularly by road,” Mr Mesaglio says. “But it means there’s an exciting opportunity to visit these locations because we might capture something that has never before been photographed.”

Activating citizen scientist snaps

It’s one thing to have comprehensive photographic records for professional scientists to use in identification guides. But when the plant world is under threat from multiple fronts, including habitat clearing and climate change, photos can help engage the public in plant science.

“People can engage with, sympathise with, and get much more excited about plants with photographs, which is vital when our natural environments are more at risk than ever,” Mr Mesaglio says.

“Because digital photography is so accessible now, anyone can also help make a meaningful contribution to science using the camera in their pocket.”

Using a platform like iNaturalist, keen citizen scientists can have their snaps identified by experts and share the data with aggregators like the Atlas of Living Australia and the Global Biodiversity Information Facility to be used in research and conservation.

“Since April last year, we’ve identified nearly 10 per cent of those previously unphotographed species thanks to members of the public uploading their photographs and experts who’ve kindly identified them,” Mr Mesaglio says. “There could be many more in personal collections or behind paywalls just waiting to be shared.”

The researchers recommend a standardised system for scientific plant photography be developed, starting with a requirement in the International Code of Nomenclature for Plants to include at least one field photograph where possible in new species descriptions. They also suggest all new species descriptions be published as Open Access in searchable databases with Creative Commons licensing to maximise their usage.

“We also suspect more photos exist, but they’re hidden away on social media or behind scientific paywalls that aren’t accessible, discoverable, or searchable,” Mr Mesaglio says.

“Of the species with photographs, many have a single photo. We not only want to capture those unrepresented species but also continue building the photographic record for all species.

“Doing so will help us identify, monitor and conserve our native species for generations to come.”

But they also tend to have good intentions, the study found.

The study, published this week in the Journal of Medical Internet Research, provides some of the first insights into the burgeoning, loosely regulated world of so-called “patient influencers,” sharing findings from 26 in-depth interviews about why and how they do it.

“The bottom line here is that patient influencers act as a form of interactive direct-to-consumer (DTC) advertising, sharing their knowledge and experiences on pharmaceutical drugs with communities of followers in which they wield great influence,” said author Erin Willis, an associate professor of advertising, public relations and media design. “This raises ethical questions that need more investigation.”

The study comes amid growing concerns about the harmful consequences of drug promotion on social media.

In recent weeks, in the wake of a slew of TikTok videos and Twitter posts touting the weight loss benefits of the diabetes drug Ozempic, patients who need the medication to manage their disease have faced global shortages. Meanwhile, those taking it “off-label” to slim down have experienced surprising side-effects, including violent diarrhea and extreme facial thinning.

“This is a great example of the power of social media and the unintended consequences,” said Willis.

A new kind of advertising

Controversial from its start in the 1980s, and still only available in the United States and New Zealand, DTC advertising enables drug companies to target consumers directly, rather than exclusively through physicians. About half of the people who ask their doctor about a drug after seeing a TV ad get it.

With trust in pharmaceutical companies and traditional media declining, drug makers are now turning to real patients as messengers, with companies like Health Union connecting them for partnerships.

Willis conducted one-on-one, hour-long Zoom interviews with influencers with a range of conditions, including lupus, fibromyalgia, Parkinson’s disease, asthma, HIV, celiac disease, chronic migraines and perimenopause. Eighteen of the 26 collaborated with a pharmaceutical company in some way.

Most had between 1,000 and 40,000 followers. Such “micro influencers” tend to be less expensive for advertisers to work with than celebrities, and research has shown they have the most influence on purchasing behaviors, said Willis.

Some interviewees posted company press releases directly. Others read studies about drugs and translated results for followers. Some were paid to post content for drug companies.

“Health literacy and digital literacy are both concerningly low in this country,” said Willis, noting that consumers often fail to recognize the difference between a sponsored ad and an altruistic personal post. “The fact that patients with no medical training are broadly sharing drug information should alarm us.”

Good intentions

On the positive side, Willis was heartened by the reasons participants become influencers.

Almost all said they were drawn to their roles by a sense that the answers they sought as patients, didn’t exist in other channels.

“I spent a lot of time looking for diabetes information that related to me — an African American woman from the South,” reported one study participant. “I didn’t see what I needed, so I created it.”

Others were motivated by a wish to destigmatize disability in certain communities.

“There’s still not a lot of talk about Latinos and HIV,” said another participant. “When there was information, it wasn’t culturally appropriate.”

Five said they never share information about drugs, stating that they believed it was “borderline unethical.”

Others said they would only post about drugs they personally had been prescribed and taken and always encouraged followers to consult with their doctor. They all said they generally strived to behave ethically.

“It’s comforting that the people we interviewed generally want to stay abreast of the science and be a credible source,” said Willis. “But I also know that doctors go to medical school for a reason.”

Concerns abound

Several influencers reported that followers frequently private message them to get detailed information about dosage and side effects.

“In an online community, there are other people there to say, ‘That’s not true or that’s not what I experienced.'” Willis said. “But with social media, a lot of the conversation happens privately.”

Willis also worries that influencers may stress the upsides of medications without fully disclosing the side-effects. For instance, she references a famously controversial 2015 post by celebrity influencer Kim Kardashian, singing the praises of a “#morningsickness” drug called Diclegis to her tens of millions of followers on Instagram.

The Food and Drug Administration swiftly flagged the post for omitting the drug’s long list of risks, required Kardashian to remove the post and dinged the drug maker with a warning letter. The Federal Trade Commission (FTC) now requires influencers to disclose whether they are paid via hashtags, such as #ad or #sponcon, and the Food and Drug Administration has rules on what can be said on social posts. But those rules are open to interpretation, and videos, disappearing content and direct messaging can be tough to track.

Willis acknowledged that her sample was a small one and that because many of her interviewees were referred to her by Health Union, they likely skew to the responsible side. In future studies, she intends to include broader sample sizes, explore how influencers impact treatment decisions and investigate compensation for and regulations around patient influencers.

Analysts predict the influencer marketing industry as a whole will be valued at $21.1 billion in 2023.

As patient influencers increasingly find their place in it, Willis contends that regulators should work harder to keep up with all the new platforms.

“This is happening, with or without regulation, and people should be aware of it,” Willis said.

The research is based on new analyses of stone tools used by long-tailed macaques in the Phang Nga National Park in Thailand. These monkeys use stone tools to crack open hard-shelled nuts. In that process, the monkeys often break their hammerstones and anvils. The resulting assemblage of broken stones is substantial and widespread across the landscape. Moreover, many of these artefacts bear all of the same characteristics that are commonly used to identify intentionally made stone tools in some of the earliest archaeological sites in East Africa.

“The ability to intentionally make sharp stone flakes is seen as a crucial point in the evolution of hominins, and understanding how and when this occurred is a huge question that is typically investigated through the study of past artefacts and fossils. Our study shows that stone tool production is not unique to humans and our ancestors,” says lead author Tomos Proffitt, a researcher at the Max Planck Institute for Evolutionary Anthropology. “The fact that these macaques use stone tools to process nuts is not surprising, as they also use tools to gain access to various shellfish as well. What is interesting is that, in doing so they accidently produce a substantial archaeological record of their own that is partly indistinguishable from some hominin artefacts.”

New insights into the evolution of stone tool technology

By comparing the accidentally produced stone fragments made by the macaques with those from some of the earliest archaeological sites, the researchers were able to show that many of the artefacts produced by monkeys fall within the range of those commonly associated with early hominins. Co-lead author Jonathan Reeves highlights: “The fact that these artifacts can be produced through nut cracking has implications for the range of behaviours we associate with sharp edged flakes in the archaeological record..”

The newly discovered macaque stone tools offer new insights into how the first technology might have started in our earliest ancestors and that its origin may have been linked to similar nut cracking behaviour which could be substantially older than the current earliest archaeological record. “Cracking nuts using stone hammers and anvils, similar to what some primates do today, has been suggested by some as a possible precursor to intentional stone tool production. This study, along with previous ones published by our group, opens the door to being able to identify such an archaeological signature in the future,” says Lydia Luncz, senior author of the study and head of the Technological Primates Research Group at the Max Planck Institute for Evolutionary Anthropology. “This discovery shows how living primates can help researchers investigate the origin and evolution of tool use in our own lineage.”

The research, published in Science Advances, has important implications in the search for extraterrestrial life because it gives researchers a new clue to look for, said Vikas Nanda, a researcher at the Center for Advanced Biotechnology and Medicine (CABM) at Rutgers.

Based on laboratory studies, Rutgers scientists say one of the most likely chemical candidates that kickstarted life was a simple peptide with two nickel atoms they are calling “Nickelback” not because it has anything to do with the Canadian rock band, but because its backbone nitrogen atoms bond two critical nickel atoms. A peptide is a constituent of a protein made up of a few elemental building blocks known as amino acids.

“Scientists believe that sometime between 3.5 and 3.8 billion years ago there was a tipping point, something that kickstarted the change from prebiotic chemistry — molecules before life — to living, biological systems,” Nanda said. “We believe the change was sparked by a few small precursor proteins that performed key steps in an ancient metabolic reaction. And we think we’ve found one of these ‘pioneer peptides’.”

The scientists conducting the study are part of a Rutgers-led team called Evolution of Nanomachines in Geospheres and Microbial Ancestors (ENIGMA), which is part of the Astrobiology program at NASA. The researchers are seeking to understand how proteins evolved to become the predominant catalyst of life on Earth.

When scouring the universe with telescopes and probes for signs of past, present or emerging life, NASA scientists look for specific “biosignatures” known to be harbingers of life. Peptides like nickelback could become the latest biosignature employed by NASA to detect planets on the verge of producing life, Nanda said.

An original instigating chemical, the researchers reasoned, would need to be simple enough to be able to assemble spontaneously in a prebiotic soup. But it would have to be sufficiently chemically active to possess the potential to take energy from the environment to drive a biochemical process.

To do so, the researchers adopted a “reductionist” approach: They started by examining existing contemporary proteins known to be associated with metabolic processes. Knowing the proteins were too complex to have emerged early on, they pared them down to their basic structure.

After sequences of experiments, researchers concluded the best candidate was Nickelback. The peptide is made of 13 amino acids and binds two nickel ions.

Nickel, they reasoned, was an abundant metal in early oceans. When bound to the peptide, the nickel atoms become potent catalysts, attracting additional protons and electrons and producing hydrogen gas. Hydrogen, the researchers reasoned, was also more abundant on early Earth and would have been a critical source of energy to power metabolism.

“This is important because, while there are many theories about the origins of life, there are very few actual laboratory tests of these ideas,” Nanda said. “This work shows that, not only are simple protein metabolic enzymes possible, but that they are very stable and very active — making them a plausible starting point for life.”

To overcome the resolution limitation, various super-resolution microscopy techniques based on diverse principles have been proposed. Yet these techniques commonly acquire super-resolution at the expense of reduced imaging speed, so achieving high-speed super-resolution imaging that can detect fast dynamics with fine structures has remained a great challenge.

Recently, a research team from East China Normal University, Shenzhen University, and Peking University resolved the contradiction between the spatial resolution and imaging speed. As reported in Advanced Photonics, they achieved high-speed super-resolution by developing an effective technique termed temporal compressive super-resolution microscopy (TCSRM). TCSRM merges enhanced temporal compressive microscopy with deep-learning-based super-resolution image reconstruction. Enhanced temporal compressive microscopy improves the imaging speed by reconstructing multiple images from one compressed image, and the deep-learning-based image reconstruction achieves the super-resolution effect without reduction in imaging speed. Their iterative image reconstruction algorithm contains motion estimation, merging estimation, scene correction, and super-resolution processing to extract the super-resolution image sequence from compressed and reference measurements.

Their studies verified the high-speed super-resolution imaging ability of TCSRM in theory and experiment. To demonstrate the imaging capability of TCSRM, they imaged flowing fluorescent beads in a microchannel, achieving a remarkable frame rate of 1200 frames per second and spatial resolution of 100 nm.

According to corresponding author Shian Zhang, Professor and Deputy Director of the State Key Laboratory of Precision Spectroscopy at East China Normal University, “This work provides a powerful tool for the observation of high-speed dynamics of fine structures, especially in hydromechanics and biomedical fields, such as microflow velocity measurement, organelle interactions, intracellular transports and neural dynamics.” Zhang adds, “The framework of TCSRM can also offer guidance for achieving higher imaging speed and spatial resolution in holography, coherent diffraction imaging, and fringe projection profilometry.”