A research team led by Colorado State University (CSU) and funded by the Climate and Land Use Alliance found that widespread use of improved ranching management practices in two distinct areas of beef production would lead to substantial emissions reductions. This includes increased efficiency to produce more beef per unit of GHG emitted — growing bigger cows at a faster rate — and enhanced land management strategies to increase soil and plant carbon sequestration on grazed lands.

Globally, cattle produce about 78% of total livestock GHG emissions. Yet, there are many known management solutions that, if adopted broadly, can reduce, but not totally eliminate, the beef industry’s climate change footprint, according to lead author Daniela Cusack, an assistant professor in the Department of Ecosystem Science and Sustainability at CSU.

Overall, the research team found a 46% reduction in net GHG emissions per unit of beef was achieved at sites using carbon sequestration management strategies on grazed lands, including using organic soil amendments and restoring trees and perennial vegetation to areas of degraded forests, woodlands and riverbanks. Additionally, researchers found an overall 8% reduction in net GHGs was achieved at sites using growth efficiency strategies. Net-zero emissions, however, were only achieved in 2% of studies.

“Our analysis shows that we can improve the efficiency and sustainability of beef production, which would significantly reduce the industry’s climate impact,” said Cusack, also a research associate at the Smithsonian Tropical Research Institute in Panama. “But at the same time, we will never reach net-zero emissions without further innovation and strategies beyond land management and increased growth efficiency. There’s a lot of room, globally, for improvement.”

Global analysis

Researchers analyzed 292 comparisons of “improved” versus “conventional” beef production systems across Asia, Australia, Brazil, Canada, Latin America and the U.S. The analysis revealed that Brazilian beef production holds the most potential for emissions reductions.

In the studies analyzed, researchers found a 57% GHG emission reduction through improved management strategies for both carbon sequestration and production efficiency in Brazil. Specific strategies include improved feed quality, better breed selections and enhanced fertilizer management.

The biggest impact was found in integrated field management, including intensive rotational grazing schemes, adding soil compost, reforestation of degraded areas and selectively planting forage plants bred for sequestering carbon in soils.

“My home country of Brazil has more than 52 million hectares of degraded pastureland — larger than the state of California,” said Amanda Cordeiro, co-author and a graduate student at CSU. “If we can aim for a large-scale regeneration of degraded pastures, implementation of silvo-agro-forestry systems and adoption of other diversified local management strategies to cattle production, Brazil can drastically decrease carbon emissions.”

In the U.S., researchers found that carbon sequestration strategies such as integrated field management and intensive rotational grazing reduced beef GHG emissions by more than 100% — or net-zero emissions — in a few grazing systems. But efficiency strategies were not as successful in the U.S. studies, possibly because of a high use of the strategies in the region already.

“Our research shows the important role that ranchers can play in combatting the global climate crisis, while ensuring their livelihoods and way of life,” said Clare Kazanski, co-author and North America region scientist with The Nature Conservancy. “By analyzing management strategies in the U.S. and around the world, our research reinforces that ranchers are in a key position to reduce emissions in beef production through various management strategies tailored to their local conditions.”

Darrell Wood, a northern California rancher, is an example of a producer leading the way on climate-friendly practices. Wood’s family participates in the California Healthy Soils program, which incentivizes practices with a demonstrated climate benefit.

“As a sixth-generation cattle rancher, I see nothing but upside potential from using our cattle as a tool for reducing greenhouse gas emissions,” Wood said. “Taking good care of our grasslands not only benefits climate, but also wildlife and the whole ecosystem that generates clean air and water. It’ll help the next generation continue our business, too.”

Next steps

Although the research shows a significant reduction in the GHG footprints of beef production using improved management strategies, scientists don’t yet know the full potential of shifting to these emission-reducing practices because there are very few data on practice adoption levels around the world.

“Asia, for example, is one of the most rapidly growing beef markets, but there is an imbalance between the amount of research focus on improving beef production and the growing demand for beef,” Cusack said. “We know with the right land management and efficiency strategies in place, it’s possible to have large reductions in emissions across geographic regions, but we need to keep pushing for additional innovations to create a truly transformation shift in the way the global beef system operates to ensure a secure food supply and a healthy environment.”

Additional co-authors on the paper include Alexandra Hedgpeth, Kenyon Chow and Jason Karpman (University of California, Los Angeles); and Rebecca Ryals (University of California, Merced).

The researchers will present their results today at the spring meeting of the American Chemical Society (ACS).

Each year, the World Anti-Doping Agency (WADA) publishes a list of substances, including steroids, that athletes are prohibited from using. However, it can be difficult to distinguish an athlete’s natural or “endogenous” steroids from synthetic “exogenous” ones administered to boost performance.

And regulatory bodies face another challenge: “As quickly as we develop methods to look for performance-enhancing drugs, clandestine labs develop new substances that give athletes a competitive advantage,” says Christopher Chouinard, Ph.D., the project’s principal investigator. Those designer drugs evade detection if testing labs don’t know to look for their specific chemical structures.

Chouinard’s team at Florida Institute of Technology is trying to outsmart cheaters with an assay that can differentiate endogenous and exogenous steroids and can also anticipate the structure of new compounds that might show up in athletes’ urine samples.

Currently, testing labs analyze samples using tandem mass spectrometry (MS) and gas or liquid chromatography. These approaches break up molecules in the sample and separate the fragments, yielding spectra that can reveal the identity of the original, intact compounds. But it can be tough to differentiate molecules with minor structural differences — including isomers — that distinguish endogenous steroids from exogenous ones, such as the synthetic anabolic steroids athletes take to build muscle.

To accentuate those differences, Chouinard pairs MS with ion mobility (IM) spectrometry, a separation technique he learned as a graduate student with Richard Yost, Ph.D., at the University of Florida. Yost’s team and others found that the differences between isomers could be made even more apparent if the molecules in a sample were modified prior to IM-mass spec analysis by reacting them with other compounds. After Chouinard set up his own lab in 2018, he applied this technique by reacting steroid samples with ozone or acetone in the presence of ultraviolet light — reactions already well-established among researchers who study lipid isomers, but new in the anti-doping arena.

Last year, Chouinard’s team reported they had successfully used these reactions with IM-MS to improve isomer separation, identification and quantification for a few steroids in sample solutions. Now, the researchers report they have tested this technique in urine against nearly half the prohibited steroids on WADA’s list and have shown it can successfully characterize and identify these compounds. They also showed the method can characterize and identify banned glucocorticoids, such as cortisone, that improve athletic performance by suppressing inflammation from injuries. Detection limits are below one nanogram per ml.

In addition to tracking down known dopants, the team wants to be able to find newly created illicit steroids not yet known to WADA. With Florida Institute of Technology collaborators including Roberto Peverati, Ph.D., they are developing computational modeling and machine learning techniques to try to predict the structure, spectra and other characteristics of these molecules. “If we can develop methods to identify any theoretical steroids in the future, we could dramatically reduce doping because we would be able to detect these new species immediately, without the lag time that’s been associated with anti-doping testing over the last 40 years,” Chouinard says.

Though the assays themselves are quick, simple and inexpensive, IM instruments are costly, with a price ranging up to roughly a million dollars, Chouinard notes. However, he adds, with the support of anti-doping funding organizations like the Partnership for Clean Competition (PCC), more labs might be willing to foot that bill, so long as the method offers a significant advantage in detection and deterrence.

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The researchers will present their results today at the spring meeting of the American Chemical Society (ACS).

If developed successfully, a fish-oil based polyurethane could help meet the immense need for more sustainable plastics, says Francesca Kerton, Ph.D., the project’s principal investigator. “It is important that we start designing plastics with an end-of-life plan, whether it’s chemical degradation that turns the material into carbon dioxide and water, or recycling and repurposing.”

To make the new material, Kerton’s team started out with oil extracted from the remains of Atlantic salmon, after the fish were prepared for sale to consumers. “I find it interesting how we can make something useful, something that could even change the way plastics are made, from the garbage that people just throw out,” says Mikhailey Wheeler, a graduate student who is presenting the work at the meeting. Both Kerton and Wheeler are at Memorial University of Newfoundland (Canada).

The conventional method for producing polyurethanes presents a number of environmental and safety problems. It requires crude oil, a non-renewable resource, and phosgene, a colorless and highly toxic gas. The synthesis generates isocyanates, potent respiratory irritants, and the final product does not readily break down in the environment. The limited biodegradation that does occur can release carcinogenic compounds. Meanwhile, demand for greener alternatives is growing. Previously, others have developed new polyurethanes using plant-derived oils to replace petroleum. However, these too come with a drawback: The crops, often soybeans, that produce the oil require land that could otherwise be used to grow food.

Leftover fish struck Kerton as a promising alternative. Salmon farming is a major industry for coastal Newfoundland, where her university is located. After the fish are processed, leftover parts are often discarded, but sometimes oil is extracted from them. Kerton and her colleagues developed a process for converting this fish oil into a polyurethane-like polymer. First, they add oxygen to the unsaturated oil in a controlled way to form epoxides, molecules similar to those in epoxy resin. After reacting these epoxides with carbon dioxide, they link the resulting molecules together with nitrogen-containing amines to form the new material.

But does the plastic smell fishy? “When we start the process with the fish oil, there is a faint kind of fish smell, but as we go through the steps, that smell disappears,” Kerton says.

Kerton and her team described this method in a paper last August, and since then, Wheeler has been tweaking it. She has recently had some success swapping out the amine for amino acids, which simplifies the chemistry involved. And while the amine they used previously had to be derived from cashew nut shells, the amino acids already exist in nature. Wheeler’s preliminary results suggest that histidine and asparagine could fill in for the amine by linking together the polymer’s components.

In other experiments, they have begun examining how readily the new material would likely break down once its useful life is over. Wheeler soaked pieces of it in water, and to speed up the degradation for some pieces, she added lipase, an enzyme capable of breaking down fats like those in the fish oil. Under a microscope, she later saw microbial growth on all of the samples, even those that had been in plain water, an encouraging sign that the new material might biodegrade readily, Wheeler says.

Kerton and Wheeler plan to continue testing the effects of using an amino acid in the synthesis and studying how amenable the material is to the microbial growth that could hasten its breakdown. They also intend to study its physical properties to see how it might potentially be used in real world applications, such as in packaging or fibers for clothing.

The researchers will present their results today at the spring meeting of the American Chemical Society (ACS). 

Each year in the U.S., at least 2.8 million people get antibiotic-resistant infections, leading to 35,000 deaths, according to the U.S. Centers for Disease Control and Prevention. “We knew from previous research that there were substances in Baltic amber that might lead to new antibiotics, but they had not been systematically explored,” says Elizabeth Ambrose, Ph.D., who is the principal investigator of the project. “We have now extracted and identified several compounds in Baltic amber that show activity against gram-positive, antibiotic-resistant bacteria.”

Ambrose’s interest originally stemmed from her Baltic heritage. While visiting family in Lithuania, she collected amber samples and heard stories about their medicinal uses. The Baltic Sea region contains the world’s largest deposit of the material, which is fossilized resin formed about 44 million years ago. The resin oozed from now-extinct pines in the Sciadopityaceae family and acted as a defense against microorganisms such as bacteria and fungi, as well as herbivorous insects that would become trapped in the resin.

Ambrose and graduate student Connor McDermott, who are at the University of Minnesota, analyzed commercially available Baltic amber samples, in addition to some that Ambrose had collected. “One major challenge was preparing a homogeneous fine powder from the amber pebbles that could be extracted with solvents,” McDermott explains. He used a tabletop jar rolling mill, in which the jar is filled with ceramic beads and amber pebbles and rotated on its side. Through trial and error, he determined the correct ratio of beads to pebbles to yield a semi-fine powder. Then, using various combinations of solvents and techniques, he filtered, concentrated and analyzed the amber powder extracts by gas chromatography-mass spectrometry (GC-MS).

Dozens of compounds were identified from the GC-MS spectra. The most interesting were abietic acid, dehydroabietic acid and palustric acid — 20-carbon, three-ringed organic compounds with known biological activity. Because these compounds are difficult to purify, the researchers bought pure samples and sent them to a company that tested their activity against nine bacterial species, some of which are known to be antibiotic resistant.

“The most important finding is that these compounds are active against gram-positive bacteria, such as certain Staphylococcus aureus strains, but not gram-negative bacteria,” McDermott says. Gram-positive bacteria have a less complex cell wall than gram-negative bacteria. “This implies that the composition of the bacterial membrane is important for the activity of the compounds,” he says. McDermott also obtained a Japanese umbrella pine, the closest living species to the trees that produced the resin that became Baltic amber. He extracted resin from the needles and stem and identified sclarene, a molecule present in the extracts that could theoretically undergo chemical transformations to produce the bioactive compounds the researchers found in Baltic amber samples.

“We are excited to move forward with these results,” Ambrose says. “Abietic acids and their derivatives are potentially an untapped source of new medicines, especially for treating infections caused by gram-positive bacteria, which are increasingly becoming resistant to known antibiotics.”

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