Health24.com | Brave six-year-old saves grandad’s life – by feeding him jelly babies!

A little boy saved his great-grandfather’s life after he slipped into a diabetic coma.

On Saturday 21 July young Aiden Smith from Walsall, England, was visiting his great-granddad Brian Clark when he noticed the pensioner having a hypoglycaemic attack, which left him unable to speak.

But instead of panicking, the primary school pupil rushed to grab Brian’s blood-sugar testing kit after seeing the 73-year-old use it many times before.

When Aiden realised the retired engineer’s blood-sugar levels were dangerously low, he quickly grabbed some jelly babies – saving Brian from a fatal coma.

“I’m so proud of Aiden, he’s so smart,” Brian says.

“My wife was out shopping so it was just us in the house. Suddenly I realised I wasn’t well but I couldn’t move and couldn’t say a thing.

“Aiden had watched me measuring my blood sugar every day so he knew how to use it.

“Then he ran and got the jelly babies and quickly put a few in my mouth.

“He was very calm. It took me about 10 minutes to come around.

“I’m very proud, he really saved my life.”

Little Aiden usually spends the weekends with Brian and his great-grandmother Sandra (70) but on Saturday he was alone with his great-granddad.

After feeding the pensioner, who has had type 2 diabetes for 30 years, two jelly babies and giving him Lucozade to sip on, the six-year-old tested Brian’s sugar level again.

Luckily, within 10 minutes his sugar level had gone up and the great-grandad was feeling much better by the time his wife came home.

“I was really shocked,” Sandra said. “I couldn’t have done much even if I’d known what was happening as I was out of the house.

“Aiden has watched his grandad use the finger-prick tester so many times, so he knew how it worked.

“I’m just so proud of him.”

Aiden – who’s also suffered from asthma attacks – now hopes to become a paramedic.

“He’s never had any first aid training. We’ve all been watching my grandad for years and we just know what to do, so he did it automatically,” said Aiden’s mom, Hayley Farren (27).

“His brain is like a sponge, he absorbs everything.

“I don’t think he even realised how important it was what he did. He saved grandad’s life. He’d definitely be in a coma if it hadn’t been for Aiden.

“When I asked him about it he just said, ‘Mommy, I just knew what I needed to do.’

“I’m so proud of him – for a six-year-old to do all this is a big deal.

“He’s such a sweet, loving little boy. He always thinks about others more than himself.”

Source: Magazine Features

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Engineered genetic machinery derived from E. coli delivers new amino acids to proteins

Proteins — the molecular machines that drive the processes underlying biology — are made from just 20 canonical building blocks called amino acids. For nearly two decades, scientists have sought methods to engineer new amino acids to build proteins.

A team of Boston College chemists have developed a technology to precisely incorporate a range of useful non-canonical amino acids into proteins made in eukaryotes, the broad class of higher organisms that includes humans, the team reported in the journal Cell Chemical Biology.

Approximately 15 years ago, scientists first saw the potential of an engineered, bacteria-derived genetic machinery — known as an aminoacyl-tRNA synthetase/tRNA pair — for incorporating non-canonical amino acids into proteins produced in eukaryotic cells. But the method has faced a number of technical restrictions that limited its widespread development.

The Boston College team overcame many of those limitations by developing a novel strain of E. coli bacteria that enables facile engineering of the bacteria-derived aminoacyl-tRNA synthetase/tRNA pair, according to Assistant Professor of Chemistry Abhishek Chatterjee, who led the project. This new approach enabled the incorporation of various non-canonical amino acids, including p-boronophenylalanine, into proteins produced in human cells as well as in the engineered strain of E. coli.

Chatterjee said the team was surprised by the facility of the new approach, which is described in the new report “Resurrecting the bacterial tyrosyl-tRNA synthetase/tRNA pair for expanding the genetic code of both E. coli and eukaryotes.”

“Creating this novel E. coli strain required substituting its native aminoacyl-tRNA synthetase/tRNA pair with a counterpart from a different organism, which we anticipated would be very difficult,” he said. “But it turned out to be quite feasible. That opens up this complete technology.”

Chatterjee said the team sought to create a new method to engineer and monitor protein functions as a way of expanding the scientific understanding of the processes that guide protein functions in our cells.

“Thousands of proteins are encoded in the genome that make us who we are, but we know very little about that process,” said Chatterjee. “In human cells, there are roughly 20,000 protein-coding genes. What they are doing and how they are doing it remains difficult to study. One of the major problems is that if you want to know what they are doing, you have to spy on them. You need to attach a probe that can report back on what is going on.”

Introducing such probes has proven difficult, as the process often damages the target protein.

In any cell, proteins are made from 20 amino acids — a fixed group guided into order by genetic instructions.

“The idea is that we can introduce a new building block into proteins that nature does not have — beyond the 20 canonical amino acids that nature uses,” Chatterjee said. “If we can do that, we have the ability to very specifically introduce a wide variety of non-natural functionalities into any site of virtually any protein.”

The immediate benefit would be to assist researchers who are still unraveling the mysteries of cell biology and protein function.

“You could create a protein with a non-canonical amino acid into any chosen site, load it with probes that are very tiny and give out an optical signal that tells where it is going,” Chatterjee said. “It could allow you to manipulate how the protein is working. You could introduce limits, so whatever the protein is doing, it can’t do any more. And you could remove the probe by using an external signal such as light. This technology opens up numerous new ways one can start to probe and engineer protein function, which would be very challenging otherwise.”

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Materials provided by Boston College. Note: Content may be edited for style and length.

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Key gene to accelerate sugarcane growth is identified

Despite international breeding efforts, advanced agronomy and effective management of pests and diseases, sugarcane yields have been static for decades owing to constraints on culm development. The culm’s sugar storage capacity is physically limited, restricting the volume of sucrose and biomass that can be obtained from the crop for sugar and second-generation (2G) ethanol production, according to experts in the area.

“Breaking through this developmental threshold by conventional breeding [crossing of varieties] is proving hard,” said Marcelo Menossi, a Full Professor in the Department of Genetics, Evolution & Bioagents at the University of Campinas’s Biology Institute (IB-UNICAMP) in Brazil.

Menossi, in collaboration with postgraduate students supervised by him and with colleagues at Brazil’s National Bioethanol Science & Technology Laboratory (CTBE), Sugar Research Australia and Germany’s Martin Luther University Halle-Wittenberg (MLU), found that the key to surmounting this constraint on sugarcane yield could lie in a gene called ScGAI.

In a study performed during a project linked to BIOEN, the Bioenergy Research Program from FAPESP — São Paulo Research Foundation, Menossi and collaborators discovered that ScGAI is an important regulator of culm development in sugarcane.

By manipulating the activity of this gene in transgenic sugarcane lines developed in Australia, the researchers succeeded in substantially increasing culm volume and changing the allocation of carbon to structural and storage molecules. They have now reported their findings in an article published in Journal of Experimental Botany.

“The sugarcane variety in which we altered the expression of ScGAI developed far more quickly. This means it should be possible to breed a variety of energy sugarcane that matures faster and has higher biomass production per unit of time,” Menossi said.

The discovery was derived from the PhD research of Rafael Garcia Tavares, conducted at IB-UNICAMP under Menossi’s supervision and with a scholarship from FAPESP.

During the study, ScGAI was found to mediate the regulation of developmental hormones used by sugarcane growers, such as ethylene and gibberellins.

Gibberellins are widely employed to improve yield in many crops and accelerate sugarcane ripening by triggering the rapid degradation of DELLA proteins, which are thereby prevented from interacting with and degrading other proteins that stimulate culm development.

“Ethylene is typically applied to sugarcane plantations during the ripening stage, when the farmer wants the plant to continue accumulating sucrose instead of growing and producing more leaves. Unlike gibberellins, it stabilizes DELLAs and lets them interact with and degrade other growth-promoting proteins. However, the role of DELLAs as regulators of sugarcane growth was poorly understood,” Menossi said.

Patenting of manipulation method

To glean a better understanding of how DELLAs regulate sugarcane growth, especially with regard to culm development, the researchers conducted an experiment in which they altered ScGAI expression in an Australian sugarcane variety.

In some lines, ScGAI was silenced to reduce the production of DELLAs and stop them from degrading other proteins that contribute to the plant’s development.

In other lines, the researchers took steps to ensure that the gene was overexpressed with the aim of increasing the production of DELLAs and stabilizing them so that they continued to degrade the growth-promoting proteins.

In a comparative analysis performed after four months, ScGAI-overexpressing transgenic sugarcane lines displayed stunted growth, shorter internodes, and impaired energy metabolism.

In contrast, ScGAI-silenced plants were taller and exhibited rapid internode elongation, increased phytomer production, and greater carbon allocation to the stem. The phytomer is defined as a unit comprising a node and internode, its axillary buds, and an attached leaf.

“The study clearly shows that the ScGAI gene is a fundamental component of sugarcane development and can be a target for genetic manipulation to permit intervention in the speed of plant growth by regulating DELLA proteins,” said the coordinator of the FAPESP research project.

The researchers have applied for a patent on the method they invented to manipulate ScGAI in order to decrease the amount of DELLAs in sugarcane so that the plant develops faster. Two companies have expressed an interest in the technology.

“The next step is to perform field tests and see if we can obtain the same results by growing the transgenic lines with the manipulated gene in a greenhouse,” Menossi said.

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A periodic table of molecular knots

Consider a short piece of rope: could you guess which knots are more likely to form if you crumple and shake it? Synthetic chemists have long been working on a molecular version of this problem and, so far, have succeeded at synthesizing half a dozen different knots types using molecular self-assembling techniques. But which other knot types will it be possible to realise in the future? This is the challenging question that SISSA scientists, in association with the University of Padua, have tackled using computer simulations in this new work published in Nature Communications. The scientists identified a shortlist, a kind of “periodic table,” of the most designable knot types, i.e. those knots that could easily self-assemble under appropriate physical and chemical conditions. The findings, obtained with computational predictive models, are supported by the latest experimental results and ought to aid the synthesis of as yet undiscovered topologies. This study, and the increasingly predictive capability of molecular modelling techniques, can create novel possible perspectives for future advanced applications, like the construction of sophisticated molecular machines for loading and delivering a nanocargo.

Not just an intellectual challenge

“There is a growing scientific interest in molecules with complex. In this context, the possibility to design and synthesis of novel types of molecular knots is particularly appealing.” say Mattia Marenda and Cristian Micheletti, respectively first and last author of this research.

“Until recently, only few types of molecular knots had been synthesised. These were the simplest knots in mathematic tables, i.e. those having at most 5 essential crossings.” One could have thus predicted that the next knot type to be synthesized would have had six crossings. However, in a computational study of 2015, Micheletti and collaborators argued that the simplest and most designable undiscovered knot type was significantly more complex and featured as many as 8 essential crossings. This prediction was experimentally confirmed in 2017 and motivated the current study, which employed a more systematic exploration of the shapes or configurations that can be formed from identical building blocks stitched together in a string-like fashion.

Efficient and reliable simulations

“With these models, we aimed at discovering which new molecular knots types, if any, would be easiest to obtain with current synthetic chemistry techniques, particularly self-assembly. We found that these privileged knot types do exist, but are very rare. Only a dozen of different topologies are realisable among millions of simple knot types. The results of our models had an inherent simplicity” says Marenda. “The molecular weaving of these knot types is modular and highly symmetric. We used these features as selection criteria to sift the huge combinatorial space of molecular weaving patterns and obtained a shortlist of knot types expected to be easily assembled from few identical building blocks.” “The shortlist is similar to a periodic table, in that it is organised in rows and columns which reflect different aspects of the expected difficulty of practical realization,” continues Micheletti. “The results are supported by recent experiments and this suggests that the table could indeed be useful to experimental chemists for choosing the target topologies for further studies and applications.”

Nanocargo and molecular machines: possible applications

What are possible long-term results of this research? “At this time” explain Marenda and Micheletti “chemists and physicists have mostly focussed on proof-of-concept demonstrations of the design and synthesis of molecular knots. Nonetheless, interesting applicative avenues have already been suggested.” A chief example is the assembly of molecular cages: “In this case, specific substances could be nested or trapped within weavings of synthetic molecular knots. The latter could then serve as a controllable molecular machine, capable of loading or releasing a nanocargo depending on the specific physico-chemical conditions. These are interesting and appealing perspectives for possible applications in medicine or electronics.”

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Materials provided by Scuola Internazionale Superiore di Studi Avanzati. Note: Content may be edited for style and length.

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Tropical forest seeds use three strategies to survive

The oldest living seeds found on Earth germinated after resting more than 30,000 years in Arctic soils. But in the humid tropics, seeds do not last. “A long-lived seed in the tropics is probably only a few decades old. This may not seem like much time, but it is critical to reestablish trees after deforestation and ensure species’ survival,” said Camilo Zalamea, post-doctoral fellow and lead author of a new paper in Ecology from the Smithsonian Tropical Research Institute (STRI) in Panama.

Seeds of tropical pioneers, trees that reestablish a forest after logging, flood, drought or fire, need strategies to overcome the two challenges every seed faces: 1) stay alive until the right conditions to grow into a plant occur, and 2) avoid being eaten by predators and decayed by soil microbes.

Although birds, bats or the wind can spread seeds from nearby forests, many of the seedlings that start a new forest emerge from seeds long buried in the soil — that is, from the soil seed bank. “For the first time, we’re making the connection between seed dormancy — the characteristics that make it possible for a seed to wait until environmental conditions are suitable for growth, and seed defenses — the characteristics the seed uses to avoid being eaten or decayed,” said Jim Dalling, STRI research associate and professor at the University of Illinois, Urbana-Champaign.

“Overall, pioneer tree species have three strategies to survive in the seed bank. Species that adopt one of these strategies share characteristics, or “seed-defense syndromes,” Dalling said.

“For example, some seeds may protect themselves physically by producing a very hard outer coat making it difficult for animals or microorganisms to reach the living tissues inside,” said Zalamea. “Other seeds survive because they produce chemical compounds to deter predators and pathogens. And some seeds are short-lived, investing little in physical or chemical defenses, but may be protected by special microbes they select from soil.”

Defending seeds is expensive, requiring an investment of resources from the mother tree. For 16 of the most common pioneer tree species in Panama, the research team evaluated the seeds’ physical defenses, asking: How hard is it to break the seed, how thick is the seed coat, how permeable is the seed coat and how heavy is the seed? They also evaluated the seeds’ chemical defenses, by looking at the presence and abundance of protective chemicals and testing the toxicity of seed extracts.

“We found that how much a seed invests in defending itself is directly related to the time it lasts in the soil,” said Betsy Arnold, STRI research associate and professor at the University of Arizona. For example, seeds that are capable of persisting for long periods in the soil rely more on chemical defenses, whereas species with shorter persistence rely more on physical defenses. So this work helps resolve just how seeds can remain alive in the soil seed bank for months to years in tropical forests.”

Understanding how seeds evade or repel pests and diseases has broad application beyond tropical forests. Diseases of major seed crops — rice, wheat and maize — cost global agriculture billions of dollars a year in lost yield, and represent a significant threat to global food security. Nonetheless, remarkably little is known about how plants defend their seeds against enemies, or how seed defenses align with other properties of seeds.

“In our study, we found that tropical pioneer seeds defend themselves using three different dormancy-defense syndromes; we hope that this information can be used to develop more effective practices for crop production and weed management, restoring natural vegetation, and to generate a better understanding of natural plant community dynamics,” said Adam Davis, a scientist at the U.S. Department of Agriculture.

This research was funded by grants from the U.S. National Science Foundation (NSF DEB-1120205, DEB-1119758) and Simons Foundation.

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Arrests over packages sent to hospitals

Police say two men were arrested after packages containing liquid were sent to a number of hospitals.

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Engineered pig lung transplant ‘a success’

Scientists have successfully transplanted a bioengineered lung into a pig.

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Health24.com | Is it okay to drink alcohol while breastfeeding? An expert weighs in

Drinking alcohol while breastfeeding is a bit of a controversial topic, says Catherine Pereira, Association for Dietetics in South Africa spokesperson, registered dietitian and lecturer at the University of the Western Cape.

But, she warns that drinking alcohol while breastfeeding is quite different to drinking alcohol while you are pregnant.

“During pregnancy, alcohol can move across the placenta, is toxic to the developing foetus, and in high amounts can cause serious damage. With breastfeeding, the alcohol does not transfer straight into the breastmilk. Any food or drink that a mother consumes needs to be digested first, so will transfer through her whole gastrointestinal tract (stomach, intestines etc.), get broken down and then individual nutrients are absorbed into the blood,” says Pereira.

She adds that there are many factors that influence the composition of breastmilk, and the production of breastmilk is largely controlled by the hormones prolactin and oxytocin as well as the frequency (how often) a mother breastfeeds. 

So, is it safe to consume alcohol while breastfeeding?

Pereira refers to Dr Jack Newman, a renowned and recognised breastfeeding advocate, who recently compiled a Facebook post summarising his recommendations regarding breastfeeding and alcohol.

Dr Newman says, “The alcohol content in breast milk immediately after drinking is equivalent to a 0.0274 proof beverage. That’s like mixing 1 oz of 80 proof vodka (one shot) with 2 919 oz of mixer. By the way, 2 919 oz is over 70 liters [sic].” 

“The take-home message of this is that the amount of alcohol that eventually is present in breastmilk is so low that it is unlikely to affect the baby,” says Pereira. In South Africa, however, the recommendation is that if people do drink alcohol, they do so in moderation.

The National Institute on Alcohol Abuse and Alcoholism says that low-risk drinking can be defined as consuming up to one unit of alcohol per day, with no more than three drinks on a single day and no more than seven drinks per week for women; while for men it’s up to two drinks per day with no more than four drinks per day for men and then no more than 14 units of alcohol per week.

“Don’t forget, though, that what you consider to be one unit of alcohol is often much less than one drink!” says Pereira.

If you do choose to drink alcohol while breastfeeding, Pereira advises that you do so straight after a feed so that your body has the maximum amount of time to metabolise the alcohol before your baby’s next feed.   

Breastfeeding guidelines

If you are worried about the wellbeing of your baby while breastfeeding, Dr Ronald Marino, chief of the division of general paediatrics at NYU Winthrop Hospital in Mineola, New York, advises on erring on the side of caution. “Most things do get into breast milk, and we need to understand this more because we don’t know if there’s a safe exposure level for babies. But it’s probably better not to be exposed.

“Your baby’s brain is developing so much in the first year of life, you want to give them every chance to have high function. Try to stay as pure as you can when breastfeeding – [avoiding] marijuana, alcohol or even herbal remedies,” he said.

Here are some important breastfeeding guidelines, according to a previous Health24 article: 

  • Do not smoke or use any recreational drugs.
  • If you are taking medication for a chronic condition, speak to your doctor before you start breastfeeding. 
  • There are no foods that you must avoid, just ensure that you are following a balanced diet with variety. 
  • Strong-flavoured food may flavour your breastmilk but this will not affect your baby. 
  • Avoid dieting while you are breastfeeding. Pereira adds that you should lose some weight when you breastfeed.

mother and child breastfeeding factoid

10 additional messages

Pereira also points to the National Department of Health, which offers 10 important infant feeding messages:

1. During their first six months of life, the World Health Organization recommends that babies need breastmilk and nothing else (no porridge, water, tea or other foods and liquids).

2. Breastfeeding is the normal way to provide babies with food for healthy growth and development.

3. It is safe for HIV positive mothers to breastfeed their babies while using antiretroviral treatment.

4. Mothers should be supported and shown how to initiate breastfeeding within the first hour after birth.

5. Mothers need to be supported to breastfeed successfully. Support them to get the baby to latch. Proper attachment encourages release of breast milk.

6. Almost all mothers will have enough breast milk to feed their babies exclusively during the first six months.

7. Everyone should support a mother to breastfeed her baby for a better, healthier life.

8. Happy breastfeeding moms make happy employees – employers must offer them privacy and time to express at work.

9. Continue to breastfeed and start introducing freshly prepared foods (such as fish, meat, vegetables and fruits) when the baby is six months old. 

10. Babies should be taken to the clinic every month to check their growth and development.

Image credit: iStock

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Health24.com | Do animals have better hearing than humans?

There is a general perception that humans don’t hear as well as animals – mainly because they can hear many sounds we can’t.

Added to this is the fact that humans have become less dependent on their senses for survival. As hunter-gatherers, our forefathers relied on especially their sight and hearing to stay alive – which changed when humans became agriculturalists around 10 000 years ago. 

Were we just never as sharp as our animal friends, is it a case of “use it or lose it”, or are there other factors at play? 

Loudness and pitch

According to Widex, the human audible range is dependent on the pitch (high or low) and loudness of sound. We use Hertz (Hz) to measure pitch and decibels (dB) to measure loudness.

As far as pitch is concerned, human hearing starts at about 20Hz and ends at 20 000Hz, but our hearing is most sensitive between 2 000 and 5 000Hz. The pitch of a dog whistle is too high and the roar of a wind turbine too low for humans to hear.

When it comes to loudness, in our most sensitive range humans can actually hear softer sounds than dogs and cats.

We can safely be exposed to sounds ranging from 0 to 85dB. Anything louder than that can eventually damage our hearing. An ambulance siren is about 125dB, and normal human speech around 65dB.

Hearing loss usually starts by affecting our upper pitches, which means that we will have difficulty hearing sounds like birdsong and instruments, such as flutes and piccolos. By middle age our upper range can drop to between 12 000 and 14 000Hz.

Some interesting animal hearing facts

  • Dolphins do not have ears. Instead, they rely on eardrums located outside their bodies. They also use sonar, or echolocation, to enhance their already superior hearing abilities.
  • Bats also utilise echolocation to determine location and size of things in the dark. In fact, they can find small insects 9m away in pitch darkness.
  • Cats are able to hear higher frequencies than both humans and dogs. Their ear muscles also allow them to rotate their ears up to 180°.
  • Elephants are tuned into frequencies 25 times lower than humans. Their feet and trunk also have specialised receptors for detecting low-frequency vibrations.
  • Dogs can hear frequencies way above humans. That’s why they know you’re home long before anyone else.
  • Pigeons can hear sounds as low as 0.5Hz and can detect distant storms, earthquakes and even volcanoes. Pigeons are also the best navigators in the world.
  • Monkeys’ hearing is slightly less acute than that of humans at frequencies below 8 000Hz, but extends up to 45 000Hz, which is an octave above the upper human limit. 

In defence of modern humans

It is true that humans cannot detect sounds occurring at all frequencies – but neither can animals.

A study done by researchers at Binghamton University in New York found that early humans could hear better than both chimpanzees and modern humans at certain frequencies. They studied the anatomy and reconstructed the hearing capabilities of some of our ancestors who lived in South Africa.  

The researchers found that our early ancestors were especially good at hearing frequencies between 1 500 and 3 500Hz, which would include the sounds of rustling leaves – and nowadays vacuum cleaners, telephones and hard consonants.

Instead of regressing as one might expect, modern humans’ hearing ability has become even more acute in the slightly higher frequencies. In fact, as mentioned above, modern humans are proficient at hearing sounds from 2 000 to 5 000Hz, and actually fare better than other primates in that range.

Being able to excel at higher frequencies is good for “complex short-range vocal communication”. This explains why we are better at having an intimate conversation across a dinner table than grunting and howling at each other out in nature. Chimps for instance have trouble hearing some sounds we use in spoken language. 

Image credit: iStock

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Health24.com | Do I need to lower my cholesterol?

According to a Health24 review, cholesterol is a fatty substance which is essential to the human body. It forms part of the lining (membrane) of every cell; it is the basic substance used for the production of hormones; it’s needed for the production of bile for digestion – and numerous other bodily processes. 

The main causes of high cholesterol are:

  • Genetics (controllable with modern medication)
  • Diet (controllable)
  • Underlying medical conditions (thyroid and kidney disorders – partly controllable)
  • Overweight (controllable)
  • Inactive lifestyle (controllable)
  • Age and sex (uncontrollable) (Sex is biologically controlled, whereas gender is “sex” assigned to nouns or behaviour.)
  • Smoking (controllable)

When should you be worried? South Africans are particularly vulnerable to developing high cholesterol, especially taking our high obesity rate into account. 

Health24 has two tools that might be useful: 

Could I have high cholesterol? 

Is your diet cholesterol-healthy?

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