The characteristic pulsations and brightness of RR Lyrae stars make them excellent “standard candles” for measuring galactic distances. These new observations allowed the researchers to trace the outer limits of the Milky Way’s halo.

“This study is redefining what constitutes the outer limits of our galaxy,” said Raja GuhaThakurta, professor and chair of astronomy and astrophysics at UC Santa Cruz. “Our galaxy and Andromeda are both so big, there’s hardly any space between the two galaxies.”

GuhaThakurta explained that the stellar halo component of our galaxy is much bigger than the disk, which is about 100,000 light years across. Our solar system resides in one of the spiral arms of the disk. In the middle of the disk is a central bulge, and surrounding it is the halo, which contains the oldest stars in the galaxy and extends for hundreds of thousands of light years in every direction.

“The halo is the hardest part to study because the outer limits are so far away,” GuhaThakurta said. “The stars are very sparse compared to the high stellar densities of the disk and the bulge, but the halo is dominated by dark matter and actually contains most of the mass of the galaxy.”

Yuting Feng, a doctoral student working with GuhaThakurta at UCSC, led the new study and is presenting their findings in two talks at the American Astronomical Society meeting in Seattle on January 9 and 11.

According to Feng, previous modeling studies had calculated that the stellar halo should extend out to around 300 kiloparsecs or 1 million light years from the galactic center. (Astronomers measure galactic distances in kiloparsecs; one kiloparsec is equal to 3,260 light years.) The 208 RR Lyrae stars detected by Feng and his colleagues ranged in distance from about 20 to 320 kiloparsecs.

“We were able to use these variable stars as reliable tracers to pin down the distances,” Feng said. “Our observations confirm the theoretical estimates of the size of the halo, so that’s an important result.”

The findings are based on data from the Next Generation Virgo Cluster Survey (NGVS), a program using the Canada-France-Hawaii Telescope (CFHT) to study a cluster of galaxies well beyond the Milky Way. The survey was not designed to detect RR Lyrae stars, so the researchers had to dig them out of the dataset. The Virgo Cluster is a large cluster of galaxies that includes the giant elliptical galaxy M87.

“To get a deep exposure of M87 and the galaxies around it, the telescope also captured the foreground stars in the same field, so the data we used are sort of a by-product of that survey,” Feng explained.

According to GuhaThakurta, the excellent quality of the NGVS data enabled the team to obtain the most reliable and precise characterization of RR Lyrae at these distances. RR Lyrae are old stars with very specific physical properties that cause them to expand and contract in a regularly repeating cycle.

“The way their brightness varies looks like an EKG — they’re like the heartbeats of the galaxy — so the brightness goes up quickly and comes down slowly, and the cycle repeats perfectly with this very characteristic shape,” GuhaThakurta said. “In addition, if you measure their average brightness, it is the same from star to star. This combination is fantastic for studying the structure of the galaxy.”

The sky is full of stars, some brighter than others, but a star may look bright because it is very luminous or because it is very close, and it can be hard to tell the difference. Astronomers can identify an RR Lyrae star from its characteristic pulsations, then use its observed brightness to calculate how far away it is. The procedures are not simple, however. More distant objects, such as quasars, can masquerade as RR Lyrae stars.

“Only astronomers know how painful it is to get reliable tracers of these distances,” Feng said. “This robust sample of distant RR Lyrae stars gives us a very powerful tool for studying the halo and testing our current models of the size and mass of our galaxy.”

This study is based on observations obtained with MegaPrime/MegaCam, a joint project of CFHT and CEA/IRFU, at the Canada-France-Hawaii Telescope (CFHT), which is operated by the National Research Council (NRC) of Canada, the Institut National des Sciences de l’Univers of the Centre National de la Recherche Scientifique (CNRS) of France, and the University of Hawaii.

“We have identified a new trigger of brain inflammation in these disorders,” said Elizabeth Ochoa, PhD, study author from UT Health San Antonio. The finding published in Science Advances is novel for this reason, she said.

Ochoa, a recent doctoral graduate, and her mentor, Bess Frost, PhD, study senior author, are investigators with the Sam and Ann Barshop Institute for Longevity and Aging Studies, the Glenn Biggs Institute for Alzheimer’s and Neurodegenerative Diseases, and the Department of Cell Systems and Anatomy at UT Health San Antonio. Frost is the Bartell Zachry Distinguished Professor for Research in Neurodegenerative Disorders.

Alzheimer’s disease and progressive supranuclear palsy are marked by toxic deposits of a protein called tau. Their research found that tau-induced “jumping genes” — which can relocate or copy themselves to other locations in the genome — form double-stranded RNA. This abnormal RNA mimics the inflammatory trigger that is also present in viral infections.

“Transposable elements — the so-called jumping genes — are a new area of interest in understanding Alzheimer’s disease. Our study provides new insights into how they can drive the disease process in addition to their ability to jump,” Ochoa said. “These double-stranded RNAs look like a virus to the immune system even though the jumping genes are a part of our normal genome.”

The researchers detected accumulation of double-stranded RNA in postmortem brain tissue from patients with Alzheimer’s disease and progressive supranuclear palsy and in brains of mouse and fruit fly models of tauopathy.

“We found substantial deposits of double-stranded RNA in astrocytes, which are cells that provide metabolic support for neurons, regulate neurotransmitters and maintain blood-brain barrier integrity,” Frost said. “In aging and disease, astrocytes respond to injury and disruption of the neuronal environment. Our findings open new doors for understanding astrocyte biology and their role in transposable element control.”

Loss of neurons, which are cells of the central nervous system, is progressive in Alzheimer’s and other neurodegenerative diseases.

The researchers conducted experiments in fruit flies to quickly test their questions about double-stranded RNA and inflammation in the brain. “To ensure that what we found in our fruit fly experiments is relevant to mammalian disease, we also studied brain tissue from mouse models and from postmortem human brains affected by tauopathy,” Ochoa said.

“As we are currently targeting jumping gene activation in a local Phase II clinical trial for patients with Alzheimer’s disease, it’s important to understand the full repertoire of toxic molecules, including double-stranded RNAs, that jumping genes produce,” Frost said.

Ochoa recently earned her PhD from the Cell Biology, Genetics and Molecular Medicine Discipline of the Integrated Biomedical Sciences program at UT Health San Antonio. She earned her undergraduate degree from Seattle University.

Frost, associate professor of cell systems and anatomy in the Barshop and Biggs institutes at UT Health San Antonio, received the highly competitive Edith and Peter O’Donnell Award in Medicine in 2020 from TAMEST, the Texas Academy of Medicine, Engineering, Science and Technology. She was also a Gold Medalist in 2022 for the Oskar Fischer Prize.

Acknowledgments

This study was supported by the National Institute of Neurological Diseases and Stroke, the Rainwater Foundation and the National Institute of General Medical Sciences. Coauthor Kevin Bieniek, PhD, is the director of the UT Health San Antonio Glenn Biggs Institute Brain Bank, which is supported by the National Institute on Aging, the Texas Alzheimer’s Research and Care Consortium, the Bill and Rebecca Reed Precision Medicine Center and the Bartell and Mollie Zachry Endowment for Alzheimer’s Research and Patient Care.

The Mayo Clinic Brain Bank provided human brain tissue. Coauthor William Ray, PhD, of the Neurodegeneration Consortium, Therapeutics Discovery Division at The University of Texas MD Anderson Cancer Center provided mouse brain tissue for analyses.

These cells assemble and maintain their own smaller, internal bits: the membrane-bound organelles like nuclei, which store genetic information, or mitochondria, which produce chemical energy. But much remains to be learned about how they organize themselves into these spatial compartments.

Physicists at Washington University in St. Louis conducted new experiments that show that eukaryotic cells can robustly control average fluctuations in organelle size. By demonstrating that organelle sizes obey a universal scaling relationship that the scientists predict theoretically, their new framework suggests that organelles grow in random bursts from a limiting pool of building blocks.

The study was published Jan. 6 in Physical Review Letters.

“In our work, we suggest that the steps by which organelles are grown — far from being an orderly ‘brick-by-brick’ assembly — occur in stochastic bursts,” said Shankar Mukherji, assistant professor of physics in Arts & Sciences.

“Such bursts fundamentally limit the precision with which organelle size is controlled but also maintain noise in organelle size within a narrow window,” Mukherji said. “Burstlike growth provides a general biophysical mechanism by which cells can maintain, on average, reliable yet plastic organelle sizes.”

Organelles must be flexible enough to allow cells to grow or shrink them as environments demand. Still, the size of organelles must be maintained within certain limits. Biologists have previously identified certain molecular factors that regulate organelle sizes, but this study provides new insights into the quantitative principles underlying organelle size control.

While this study used budding yeast as a model organism, the team is excited to explore how these assembly mechanisms are utilized across different species and cell types. Mukherji said that they plan to examine what these patterns of robustness can teach us about how to harness organelle assembly for bioengineering applications and how to spot defects in organelle biogenesis in the context of disease.

“The pattern of organelle size robustness is shared between budding yeast and human iPS cells,” Mukherji said. “The underlying molecular mechanisms producing these bursts are yet to be fully elucidated and are likely to be organelle-specific and potentially species-specific.”

Funding: This research was supported by the National Institutes of Health (NIH R35GM142704).

Story Source:

Materials provided by Washington University in St. Louis. Original written by Talia Ogliore. Note: Content may be edited for style and length.

A team of researchers, led by Young-Shin Jun, professor of energy, environmental & chemical engineering in the McKelvey School of Engineering at Washington University in St. Louis, analyzed how light breaks down polystyrene, a nonbiodegradable plastic from which packing peanuts, DVD cases and disposable utensils are made. In addition, they found that nanoplastic particles can play active roles in environmental systems. In particular, when exposed to light, the nanoplastics derived from polystyrene unexpectedly facilitated the oxidation of aqueous manganese ions and formation of manganese oxide solids that can affect the fate and transport of organic contaminants in natural and engineering water systems.

The research, published in ACS Nano Dec. 27, 2022, showed how the photochemical reaction of nanoplastics through light absorption generates peroxyl and superoxide radicals on nanoplastic surfaces, and initiates oxidation of manganese into manganese oxide solids.

“As more plastic debris accumulates in the natural environment, there are increasing concerns about its adverse effects,” said Jun, who leads the Environmental Nanochemistry Laboratory. “However, in most cases, we have been concerned about the roles of the physical presence of nanoplastics rather than their active roles as reactants. We found that such small plastic particles that can more easily interact with neighboring substances, such as heavy metals and organic contaminants, and can be more reactive than we previously thought.”

Jun and her former student, Zhenwei Gao, who earned a doctorate in environmental engineering at WashU in 2022 and is now a postdoctoral scholar at the University of Chicago, experimentally demonstrated that the different surface functional groups on polystyrene nanoplastics affected manganese oxidation rates by influencing the generation of the highly reactive radicals, peroxyl and superoxide radicals. The production of these reactive oxygen species from nanoplastics can endanger marine life and human health and potentially affects the mobility of the nanoplastics in the environment via redox reactions, which in turn might negatively impact their environmental remediation.

The team also looked at the size effects of polystyrene nanoplastics on manganese oxidation, using 30 nanometer, 100 nanometer and 500 nanometer particles. The two larger-sized nanoparticles took longer to oxidize manganese than the smaller particles. Eventually, the nanoplastics will be surrounded by newly formed manganese oxide fibers, which can make them easily aggregated and can change their reactivities and transport.

“The smaller particle size of the polystyrene nanoplastics may more easily decompose and release organic matter because of their larger surface area,” Jun said. “This dissolved organic matter may quickly produce reactive oxygen species in light and facilitate manganese oxidation.”

“This experimental work also provides useful insights into the heterogeneous nucleation and growth of manganese oxide solids on such organic substrates, which benefits our understanding of manganese oxide occurrences in the environment and engineered materials syntheses,” Jun said. “These manganese solids are excellent scavengers of redox-active species and heavy metals, further affecting geochemical element redox cycling, carbon mineralization and biological metabolisms in nature.”

Jun’s team plans to study the breakdown of diverse common plastic sources that can release nanoplastics and reactive oxidizing species and to investigate their active roles in the oxidation of transition and heavy metal ions in the future.

Funding for this research was partially provided by the National Science Foundation (CHE-1905077) and the McDonnell International Scholars Academy at Washington University in St. Louis.

Story Source:

Materials provided by Washington University in St. Louis. Original written by Beth Miller. Note: Content may be edited for style and length.