Escalating resistance by these mutants forces farmers and vector control specialists to ramp up use of poisonous compounds at increasing frequencies and concentrations, posing risks to human health and damage to the environment since most insecticides kill both ecologically important insects as well as pests.

To help counter these problems, researchers recently developed powerful technologies that genetically remove insecticide-resistant variant genes and replace them with genes that are susceptible to pesticides. These gene-drive technologies, based on CRISPR gene editing, have the potential to protect valuable crops and vastly reduce the amount of chemical pesticides required to eliminate pests.

Still, gene-drive systems have come under scrutiny with concerns that once they are released into a population they could continuously spread unchecked.

University of California San Diego geneticists have now developed a solution to this concern. Publishing in the journal Nature Communications, School of Biological Sciences Postdoctoral Scholar Ankush Auradkar and Professor Ethan Bier led the creation of a new genetic system that converts insecticide-resistant forms of mutated insect genes back to their natural, native form. The novel system is designed to spread the original “wild type” version of the gene using the biased inheritance of specific genetic variants known as alleles and then disappear, leaving only a population of insects with the corrected version of the gene.

“We have developed an efficient biological approach to reverse insecticide resistance without creating any other perturbation to the environment,” said Bier, a professor in the Department of Cell and Developmental Biology, of the self-eliminating allelic drive, or “e-Drive.” “The e-Drive is programmed to act transiently and then disappear from the population.”

As described in the paper, the researchers created a novel genetic “cassette,” a small group of DNA elements, and inserted it inside fruit flies as a proof-of-concept technology that could be applied to other insects. They developed the e-Drive to target a gene known as the voltage gated sodium ion channel, or vgsc, which is required for proper nervous system functioning.

The e-Drive cassette is designed to spread through CRISPR gene editing and features a guide RNA that binds to a Cas9 DNA protein and makes a cut at the targeted vgsc insecticide resistant gene site. The gene is then switched out for a native copy of the gene that is susceptible to insecticides.

Per the study, when insects carrying the cassette are introduced into a target population, they mate randomly and transmit the e-Drive cassette to their offspring. To maintain control of the e-Drive’s spread, the researchers imposed a fitness check on those carrying the cassette, either through limited viability or fertility. The cassette was inserted on the X-chromosome and reduced the mating success of males, resulting in reduced offspring. The frequency of the cassette in the population eventually declines through each generation until it fully vanishes from the population.

In laboratory experiments all of the offspring were converted to native genes in eight-to-10 generations, which took about six months in flies.

“Because insects carrying the gene cassette are penalized with a severe fitness cost, the element is rapidly eliminated from the population, lasting only as long as it takes to convert 100 percent of the insecticide-resistant forms of the target gene back to wild-type,” said Auradkar.

The researchers note that the self-eliminating nature of the e-Drive means it can be introduced and re-introduced as needed, and as different types of pesticides are used. The researchers are now developing a similar e-Drive system in mosquitoes to help prevent the spread of malaria.

In addition to Auradkar and Bier, the coauthors of the Nature Communications paper included their close collaborators Rodrigo Corder of the Institute of Biomedical Science, University of SãoPaulo; and John Marshall of the Innovative Genomics Institute, who performed sophisticated mathematical modeling that revealed important hidden features of the e-Drive system, including its ability to efficiently cull a class of individuals in which the drive process did not occur.

Led by Lianyi Chen, an associate professor of mechanical engineering at UW-Madison, the team discovered the mechanisms and identified the processing conditions that can lead to this significant reduction in defects. The researchers detailed their findings in a paper published on November 16, 2024, in the International Journal of Machine Tools and Manufacture.

“Previous research has normally focused on reducing one type of defect, but that would require the usage of other techniques to mitigate the remaining types of defects,” Chen says. “Based on the mechanisms we discovered, we developed an approach that can mitigate all the defects — pores, rough surfaces and large spatters — at once. In addition, our approach allows us to produce a part much faster without any quality compromises.”

Multiple industries, including aerospace, medical and energy, are increasingly interested in using additive manufacturing, also known as 3D printing, to produce metal parts with complex shapes that are difficult or impossible to create using conventional methods.

But the big challenge is that metal parts created with additive manufacturing have defects — like pores, or “voids,” rough surfaces and large spatters — that significantly compromise the finished part’s reliability and durability. These quality problems prevent 3D-printed parts from being used for critical applications where failure is not an option.

By providing a path for simultaneously increasing part quality and manufacturing productivity, the UW-Madison team’s advance could lead to widespread industry adoption of laser powder bed fusion.

Laser powder bed fusion uses a high-energy laser beam to melt and fuse thin layers of metal powder, constructing a part layer by layer from the bottom up. In this research, the UW-Madison team used an innovative ring-shaped laser beam, provided by a leading laser company called nLight, instead of the usual Gaussian-shaped beam.

The ring-shaped laser beam played a key role in this breakthrough — as did critical “in-situ” experiments, says Jiandong Yuan, the lead author of the paper and a PhD student in Chen’s group.

To see how the material behaved within the part as it was printing, researchers went to the Advanced Photon Source, an ultra-bright, high-energy synchrotron X-ray user facility at Argonne National Laboratory. Combining high-speed synchrotron X-ray imaging, theoretical analysis and numerical simulation, the researchers revealed the defect mitigation mechanisms, which involve phenomena that reduce instabilities in the laser powder bed fusion process.

The researchers also demonstrated that they could use the ring-shaped beam to drill deeper into the material without causing instabilities in the process. This enabled them to print thicker layers, increasing the manufacturing productivity. “Because we understood the underlying mechanisms, we could more quickly identify the right processing conditions to produce high-quality parts using the ring-shaped beam,” says Chen.

Lianyi Chen is the Kuo K. & Cindy F. Wang Associate Professor of mechanical engineering.

Collaborators from UW-Madison include Qilin Guo, Luis Escano, Ali Nabba, Minglei Qu, Junye Huang, Qingyuan Li, Allen Jonathan Román, and Professor Tim Osswald. Samuel Clark and Kamel Fezzaa from Argonne National Laboratory also collaborated on this project.

This work was supported by the National Science Foundation and the Wisconsin Alumni Research Foundation.

The study, published in Nature Communications, shows that Indigenous political and social networks and land use practices played key roles in the peach’s adoption and dispersal across the continent, according to the researchers.

“Peaches need a lot of care by people to be productive. They need to be planted in appropriate places with a lot of sunlight and the right soil drainage, and they need to be pruned,” said Jacob Holland-Lulewicz, first author and assistant professor of anthropology at Penn State. “For a long time, the narrative was that the Spanish introduced peaches and then peaches spread very quickly. The reality is way more complicated. How quickly peaches spread is very much a product of Indigenous networks and land management.”

The researchers analyzed historical documents that mentioned peaches, such as the travel writings of French missionary explorer Jacques Marquette and English merchant Jonathan Dickinson. They also employed radiocarbon dating — a method that measures the decay of radioactive carbon-14 atoms in organic material — to determine the approximate ages of peach pits and other organic samples, like carbonized tree wood, from 28 archaeological sites and two regional locales where archaeologists previously recovered preserved peach pits. The sites were located in the Carolinas, Georgia, Florida, Alabama, Tennessee and Arkansas.

The team found that peaches were likely widespread across Indigenous settlements in the interior southeast as early as the year 1620, roughly 100 years after the earliest Spanish expeditions in Florida and in Georgia’s Oconee Valley. The timing suggests that early Spanish settlements becoming important trade nodes within existing Indigenous networks created the necessary conditions for the spread of peaches, according to Holland-Lulewicz.

“Many narratives talk about the Spanish, or Europeans generally, arriving and then you see instantaneous changes to Indigenous histories and the spread of materials, but those initial interactions didn’t cause major changes,” he said. “It’s not until Spanish networks and Indigenous networks become entangled 100 years later that we have the necessary conditions for the spread of peaches.”

The team also identified what are possibly the earliest peaches in North America at a Muskogean farmstead in the Oconee Valley. In the 1990s, the late Penn State archaeologist James Hatch recovered peach pits from the bottom of post holes that once housed support structures for the farmstead’s house. The researchers radiocarbon dated charcoal, nuts and corn kernels from these post holes and found that occupation at the site began between 1520 and 1550 and ended between 1530 and 1570. This timing suggests that peaches had spread to the interior southeast possibly decades before the founding of St. Augustine in 1565, according to the researchers.

“Understanding the path that the introduction of species, such as peach trees, took through colonization and the role that Indigenous people and their long-term relationship with the environment played in shaping these histories demonstrates the importance of these events, people and processes to what becomes a broader American history,” said co-author Victor Thompson, Distinguished Research Professor of archaeology at the University of Georgia (UGA) and executive director of the Georgia Museum of Natural History. “Further, the fact that all of this work took place on museum specimens underscores the importance of maintaining these collections for future study.”

Indigenous peoples not only adopted the peach but selectively bred new varieties outnumbering the varieties found in Europe even at this early time, Holland-Lulewicz said.

“When Europeans started to move through and into the interior of the continent in the mid- to late 1600s, they noted that there were way more varieties of peaches being grown by Indigenous peoples than there were in Europe,” he said, explaining that the fruit had become an important aspect of Indigenous culture. “At this time, Europeans are noting really dense peach orchards around Indigenous towns, but some of these towns and people had never previously interacted with or even heard of Europeans. In fact, there are records of Indigenous peoples describing peaches as an Indigenous fruit.”

The fruit had become so integral to Indigenous history and culture that when the ancestors of the modern-day Muscogee (Creek) Nation were forcibly removed from Georgia and Alabama during the 1800s, they took peaches with them.

“There are Muscogee (Creek) peoples today who grow peaches as heritage crops,” Holland-Lulewicz said. “The act of growing and caring for those peaches is an important cultural practice. These were the first peaches introduced in the 1500s and 1600s that were then carried halfway across the continent and continue to be grown today.”

In addition to Holland-Lulewicz and Thompson, other collaborators include Amanda Roberts Thompson and Mark Williams at the UGA Laboratory of Archaeology, and Dario J. Chavez, University of Georgia; RaeLynn Butler, the Secretary of Culture and Humanities for the Muscogee (Creek) Nation, and Turner Hunt, Muscogee (Creek) Nation citizen; Jay Franklin, Logan Simpson Design; and John Worth, University of West Florida.

The UGA Laboratory of Archaeology and the Institute of Energy and the Environment at Penn State supported this work.

As part of an effort to overcome the long-term energy-storage challenge, University of Wisconsin-Madison engineers have invented a water-soluble chemical additive that improves the performance of a type of electrochemical storage called a bromide aqueous flow battery.

“Bromide-based aqueous flow batteries are a promising solution, but there are many messy electrochemical problems with them. That’s why there’s no real successful bromide-based products today,” says Patrick Sullivan who graduated from UW-Madison with a PhD in chemistry in 2023. “Yet, our one additive can solve so many different problems.”

Sullivan, PhD student Gyohun Choi, and Dawei Feng, an assistant professor of materials science and engineering at UW-Madison, developed the additive. The research was published on October 23, 2024, by the journal Nature.

Currently, giant tractor-trailer-sized lithium-ion battery packs store energy for the grid — but with technical limitations. Lithium batteries have safety concerns due to the potential for fires and explosions and a complicated international supply chain.

Aqueous flow batteries, however, could make grid-scale storage safer and cheaper. In these batteries, positive and negative liquid electrolytes circulate over electrodes that are separated by a membrane. Since the batteries use ions dissolved in a liquid — water — they can be scalable, sustainable and safe.

The most commercially mature flow batteries are based on vanadium ions, which, like lithium, are expensive and hard to source. However, another version of these flow batteries relies on bromide, a cheap, widely available ion that performs similar to vanadium — at least on paper.

In practice, however, tiny bromide ions cause all sorts of problems in flow batteries. They can pass through the membrane that separates the electrodes, and that reduces the battery’s efficiency. Sometimes the ions precipitate out of the electrolyte and form a messy oil that “sinks” to the bottom of the solution. Occasionally, the ions also form toxic bromine gas. These issues hinder practical performance and reliability.

An additive called a complexing agent could help. Choi set out to find an additive that enhances bromide aqueous flow battery performance. The researchers used molecular design to engineer over 500 candidate organic molecules they call “soft-hard zwitterionic trappers.” They synthesized and tested 13 of these representative molecules as potential additives for the bromide batteries.

The resulting multi-functional additives solve the flow battery’s main problems. It encapsulates the bromide ions while allowing them to remain water-soluble, and since the resulting complex is now larger, they can’t pass through the membrane. The ions are also “phase-stable,” which means they don’t separate out of the water electrolyte or create toxic bromine gas.

Importantly, the additives dramatically improve the flow battery’s performance, increasing the efficiency and longevity of the chemical system. “Our devices with the additive functioned without decay for almost two months compared to ones without it, which typically fail within a day,” says Feng. “This is important because for green energy storage, you want to use it for 10 or 20 years.”

The team plans to continue refining the work. Choi will study the fundamental science behind additives for bromide and iodide flow batteries, while Sullivan, who is CEO of Flux XII — a renewable energy spinoff company he co-founded with Feng — will explore the commercial viability of the additive, which has already been successfully produced in industrial ton-scale reactions.

Dawei Feng is the Y. Austin Chang Assistant Professor in materials science and engineering. Other UW-Madison authors include Xiu-Liang Lv, Wenjie Li, Kwanpyung Lee, Haoyu Kong, Sam Gessler, and JR Schmidt.

The study analysed a 4.45 billion-year-old zircon grain from the famous Martian meteorite NWA7034, also known as Black Beauty, and found geochemical ‘fingerprints’ of water-rich fluids.

Study co-author Dr Aaron Cavosie from Curtin’s School of Earth and Planetary Sciences said the discovery opened up new avenues for understanding ancient Martian hydrothermal systems associated with magmatism, as well as the planet’s past habitability.

“We used nano-scale geochemistry to detect elemental evidence of hot water on Mars 4.45 billion years ago,” Dr Cavosie said.

“Hydrothermal systems were essential for the development of life on Earth and our findings suggest Mars also had water, a key ingredient for habitable environments, during the earliest history of crust formation.”

“Through nano-scale imaging and spectroscopy, the team identified element patterns in this unique zircon, including iron, aluminium, yttrium and sodium. These elements were added as the zircon formed 4.45 billion years ago, suggesting water was present during early Martian magmatic activity.”

Dr Cavosie said the research showed that even though Mars’ crust endured massive meteorite impacts that caused major surface upheaval, water was present during the early Pre-Noachian period, prior to about 4.1 billion years ago.

“A 2022 Curtin study of the same zircon grain found it had been ‘shocked’ by a meteorite impact, marking it as the first and only known shocked zircon from Mars,” Dr Cavosie said.

“This new study takes us a step further in understanding early Mars, by way of identifying tell-tale signs of water-rich fluids from when the grain formed, providing geochemical markers of water in the oldest known Martian crust.”

Lead author Dr Jack Gillespie from the University of Lausanne was a Postdoctoral Research Associate at Curtin’s School of Earth and Planetary Sciences at the time of the study, which was co-authored by researchers from Curtin’s Space Science and Technology Centre, the John de Laeter Centre and the University of Adelaide, with funding from the Australian Research Council, Curtin University, University of Adelaide and the Swiss National Science Foundation.

The full study, titled ‘Zircon evidence for early hydrothermal activity on Mars’, will be published in Science Advances.

“The network of canals was designed to channel annual flood waters into source ponds for fish trapping and would have yielded enough fish to feed as many as 15,000 people year-round, conservatively,” said Eleanor Harrison-Buck, professor of anthropology and director of the Belize River East Archaeology (BREA) project. “The dates indicate that the fisheries were initially constructed by Late Archaic hunter-gatherer-fishers and continued to be used by their Formative Maya descendants (approximately 2000 BCE to 200 CE). For Mesoamerica in general, we tend to regard agricultural production as the engine of civilization, but this study tells us that it wasn’t just agriculture — it was also potential mass harvesting of aquatic species.”

Published in the journal Science Advances, the research used 26 radiocarbon dates from test excavation sites in the CTWS, which indicate that such landscape-scale wetland enhancements may have been an adaptive response to long-term climate disturbance recorded in Mesoamerica between 2200 and 1900 BCE.

“The early dates for the canals surprised us initially because we all assumed these massive constructions were built by the ancient Maya living in the nearby city centers,” said Harrison-Buck. “However, after running numerous radiocarbon dates, it became clear they were built much earlier.”

Sediment samples were collected along the walls of the excavation units and sequenced for specific elements, such as nitrogen and carbon, to look for environmental changes over time. The sediment showed a strong tropical forest dominance during that period and no evidence of crop cultivation, specifically maize. Along with a lack of any pollen from domesticated crops, there were not any signs of ditched and drained agricultural fields in the immediate area dating to that time. The multiproxy data gathered suggests the distinctive long linear zigzag channels served primarily as large-scale fish-trapping facilities.

“It seems likely that the canals allowed for annual fish harvests and social gatherings, which would have encouraged people to return to this area year after year and congregate for longer periods of time,” said Marieka Brouwer Burg, professor of anthropology at the University of Vermont and BREA co-director. “Such intensive investments in the landscape may have led ultimately to the development of the complex society characteristic of the pre-Columbian Maya civilization, which subsequently occurred in this area by around 1200 BCE.”

“Wetlands have always been a critical ecosystem for humans across the globe,” said Samantha Krause, professor of geography and environmental studies at Texas State University. “Knowing how to manage wetland resources responsibly is essential for the continued resilience of these ecosystems both in the past and today. The Archaic hunter-gatherer-fishers knew how to protect their resources and use them in a way that could sustain these habitats, not exhaust them, which explains their long-lasting occupation in this area.”

With the support of the local community, the team plans to return to Crooked Tree to investigate a larger sample of these landscape-scale modifications that they have identified across a broad area of northern Belize, hoping to more fully understand the complexity of human-wetland interactions in the past.

Other co-authors include Mark Willis, department of archaeology, Flinders University, Adelaide, South Australia; Angelina Perrotti, Palynology & Environmental Archaeology Research Lab; Monona, Wisconsin; and Katie Bailey, department of anthropology, University of Vermont.

This research was funded by a grant from the Alphawood Foundation Chicago. Additional support was provided by a collaborative research grant from the National Science Foundation. The Belize Institute of Archaeology provided an archaeological permit, granting permission to excavate in the Crooked Tree Wildlife Sanctuary. The Crooked Tree Village Council welcomed the research team and permitted them to map and excavate in the wetlands around their community.

The research, which was published in the journal Proceedings of the National Academy of Sciences (PNAS), offers a promising target for future therapies.

Hidradenitis suppurativa (HS) is an immune disease that affects up to 4% of the global population and causes painful, recurring skin lesions and inflammation, primarily in the folds of the skin. It commonly affects women of African American descent.

Shahid Mukhtar and his team — Bharat Mishra, Nilesh Kumar and graduate student YiFei Gou — used single-cell sequencing techniques to pinpoint CD2 as a key immune receptor with elevated expression on T cells and innate lymphoid cells (ILCs), including natural killer cells, in HS-affected skin tissue.

In collaboration with researchers at the University of Alabama at Birmingham, Mukhtar’s team demonstrated through organotypic skin culture experiments from HS patients that blocking CD2 led to a significant reduction in cytokine and chemokine production, along with suppression of key pathogenic gene signatures.

This finding suggests that blocking CD2 may effectively reduce the inflammatory response in HS, providing a potential new therapeutic avenue for managing symptoms and improving patient quality of life.

Gou, who has a keen interest in deep learning, a type of artificial intelligence (AI), hopes to further integrate single-cell transcriptomics with global protein-protein interactions using contextual AI. This approach aims to enhance understanding of cellular networks and disease mechanisms, pushing forward the frontiers of precision medicine for immune-related diseases like HS.

“Our integrative approach, combining single-cell data with molecular insights, shows the transformative potential of multiomics in discovering novel therapeutic targets,” Mukhtar said. “These findings deepen our understanding of HS and open new pathways for developing targeted therapies in HS and other immune-related conditions.”