By feeding worms with plastics and cultivating microbes found in their guts, researchers from NTU’s School of Civil and Environmental Engineering (CEE) and Singapore Centre for Environmental Life Sciences Engineering (SCELSE) have demonstrated a new method to accelerate plastic biodegradation.

Previous studies have shown that Zophobas atratus worms — the larvae of the darkling beetle commonly sold as pet food and known as ‘superworms’ for their nutritional value — can survive on a diet of plastic because its gut contains bacteria capable of breaking down common types of plastic. However, their use in plastics processing has been impractical due to the slow rate of feeding and worm maintenance.

NTU scientists have now demonstrated a way to overcome these challenges by isolating the worm’s gut bacteria and using them to do the job without the need for large scale worm breeding.

NTU Associate Professor Cao Bin at the School of CEE and Principal Investigator at SCELSE said, “A single worm can only consume about a couple of milligrams of plastic in its lifetime, so imagine the number of worms that would be needed if we were to rely on them to process our plastic waste. Our method eliminates this need by removing the worm from the equation. We focus on boosting the useful microbes in the worm gut and building an artificial ‘worm gut’ that can efficiently break down plastics.”

The study, published in Environment International in January, is aligned with the University’s commitment to fostering innovation and translating research into practical solutions that benefit society under its NTU2025 five-year strategic plan.

Developing an artificial worm gut

To develop their method, the NTU scientists fed three groups of superworms different plastic diets — High-density polyethylene (HDPE), Polypropylene (PP) and Polystyrene (PS) — over 30 days. The control group was fed a diet of oatmeal.

The NTU scientists selected the plastics as they are among the most common plastics in the world, used in everyday items like food boxes and detergent bottles. HDPE is a type of plastic known for its high-impact resistance, making it difficult to break down.

After feeding the worms plastic, scientists extracted the microbiomes from their gut and incubated them in flasks containing synthetic nutrients and different types of plastics, forming an artificial ‘worm gut’. Over six weeks, the microbiomes were left to grow in the flasks at room temperature.

Increase in plastic-degrading bacteria

The scientists found that compared to the control group, the flasks which contained the gut microbiomes from the plastic-fed worms showed a significant increase in plastic-degrading bacteria.

Furthermore, the microbial communities colonising the plastics in the flasks were simpler and more tailored to the specific type of plastic than the microbes found on plastics that had been fed directly to the worms . When the microbial communities are simpler and targeted to a specific type of plastic, this translates to potential for more efficient plastic degradation when used in real-life applications.

First author of the study Dr Liu Yinan, Research Fellow at the School of CEE and SCELSE, said, “Our study represents the first reported successful attempt to develop plastic-associated bacterial communities from gut microbiomes of plastic-fed worms. Through exposing the gut microbiomes to specific conditions, we were able to boost the abundance of plastic-degrading bacteria present in our artificial ‘worm gut,’ suggesting that our method is stable and replicable at scale.”

The researchers say their proof-of-concept lays the foundation for developing biotechnological approaches that use worms’ gut microbiomes to process plastic waste.

For their next steps, the researchers want to understand how the bacteria in the superworm’s gut break down the plastics at the molecular level. Understanding the mechanism will help scientists engineer plastic-degrading bacterial communities to break down plastics efficiently in the future.

The evidence, contained within an ice core, shows that in one location the ice sheet thinned by 450 metres — that’s more than the height of the Empire State Building — in just under 200 years.

This is the first evidence anywhere in Antarctica for such a fast loss of ice. Scientists are worried that today’s rising temperatures might destabilize parts of the West Antarctic Ice Sheet in the future, potentially passing a tipping point and inducing a runaway collapse. The new study, published in Nature Geoscience, sheds light on how quickly Antarctic ice could melt if temperatures continue to soar.

“We now have direct evidence that this ice sheet suffered rapid ice loss in the past,” said Professor Eric Wolff, senior author of the new study from Cambridge’s Department of Earth Sciences. “This scenario isn’t something that exists only in our model predictions and it could happen again if parts of this ice sheet becomes unstable.”

The Antarctic ice sheets, from west to east, contain enough freshwater to raise global sea levels by around 57 metres. The West Antarctic Ice Sheet is considered particularly vulnerable because much of it sits on bedrock that lies below sea level.

Model predictions suggest that a large part of the West Antarctic Ice Sheet could disappear in the next few centuries, causing sea levels to rise. Exactly when and how quickly the ice could be lost is, however, uncertain.

One way to train ice sheet models to make better predictions is to feed them with data on ice loss from periods of warming in Earth’s history. At the peak of Last Ice Age 20,000 years ago, Antarctic ice covered a larger area than today. As our planet thawed and temperatures slowly climbed, the West Antarctic Ice Sheet contracted to more or less its current extent.

“We wanted to know what happened to the West Antarctic Ice Sheet at the end of the Last Ice Age, when temperatures on Earth were rising, albeit at a slower rate than current anthropogenic warming,” said Dr Isobel Rowell, study co-author from the British Antarctic Survey. “Using ice cores we can go back to that time and estimate the ice sheet’s thickness and extent.”

Ice cores are made up of layers of ice that formed as snow fell and was then buried and compacted into ice crystals over thousands of years. Trapped within each ice layer are bubbles of ancient air and contaminants that mixed with each year’s snowfall — providing clues as to the changing climate and ice extent.

The researchers drilled a 651-metre-long ice core from Skytrain Ice Rise in 2019. This mound of ice sits at the edge of the ice sheet, near the point where grounded ice flows into the floating Ronne Ice Shelf.

After transporting the ice cores back to Cambridge at -20^oC, the researchers analysed them to reconstruct the ice thickness. First, they measured stable water isotopes, which indicate the temperature at the time the snow fell. Temperature decreases at higher altitudes (think of cold mountain air), so they were able to equate warmer temperatures with lower-lying, thinner ice.

They also measured the pressure of air bubbles trapped in the ice. Like temperature, air pressure also varies systematically with elevation. Lower-lying, thinner ice contains higher pressure air bubbles.

These measurements told them that ice thinned rapidly 8,000 years ago. “Once the ice thinned, it shrunk really fast,” said Wolff, “this was clearly a tipping point — a runaway process.”

They think this thinning was probably triggered by warm water getting underneath the edge of the West Antarctic Ice Sheet, which normally sits on bedrock. This likely untethered a section of the ice from bedrock, allowing it to float suddenly and forming what is now the Ronne Ice Shelf. This then allowed neighbouring Skytrain Ice Rise, no longer restrained by grounded ice, to thin rapidly.

The researchers also found that the sodium content of the ice (originating from salt in sea spray) increased about 300 years after the ice thinned. This told them that, after the ice thinned, the ice shelf shrunk back so that the sea was hundreds of kilometres nearer to their site.

“We already knew from models that the ice thinned at around this time, but the date of this was uncertain,” said Rowell. Ice sheet models placed the retreat anywhere between 12,000 and 5,000 years ago and couldn’t say how quickly it happened. “We now have a very precisely dated observation of that retreat which can be built into improved models,” said Rowell.

Although the West Antarctic Ice Sheet retreated quickly 8,000 years ago, it stabilized when it reached roughly its current extent. “It’s now crucial to find out whether extra warmth could destabilize the ice and cause it to start retreating again,” said Wolff.

Enter Professor Soojin Park, PhD candidate Minjun Je, and Dr. Hye Bin Son from the Department of Chemistry at Pohang University of Science and Technology (POSTECH). They have cracked the code, developing a pocket-friendly and rock-solid next-generation high-energy-density Li-ion battery system using micro silicon particles and gel polymer electrolytes. This work was published on the online pages of Advanced Science on the 17^th of January.

Employing silicon as a battery material presents challenges: It expands by more than three times during charging and then contracts back to its original size while discharging, significantly impacting battery efficiency. Utilizing nano-sized silicon (10^-9m) partially addresses the issue, but the sophisticated production process is complex and astronomically expensive, making it a challenging budget proposition. By contrast, micro-sized silicon (10^-6m) is superbly practical in terms of cost and energy density. Yet, the expansion issue of the larger silicon particles becomes more pronounced during battery operation, posing limitations for its use as an anode material.

The research team applied gel polymer electrolytes to develop an economical yet stable silicon-based battery system. The electrolyte within a lithium-ion battery is a crucial component, facilitating the movement of ions between the cathode and anode. Unlike conventional liquid electrolytes, gel electrolytes exist in a solid or gel state, characterized by an elastic polymer structure that has better stability than their liquid counterparts do.

The research team employed an electron beam to form covalent linkages between micro-silicon particles and gel electrolytes. These covalent linkages serve to disperse internal stress caused by volume expansion during lithium-ion battery operation, alleviating the changes in micro silicon volume and enhancing structural stability.

The outcome was remarkable: The battery exhibited stable performance even with micro silicon particles (5μm), which were a hundred times larger than those used in traditional nano-silicon anodes. Additionally, the silicon-gel electrolyte system developed by the research team exhibited ion conductivity similar to conventional batteries using liquid electrolytes, with an approximate 40% improvement in energy density. Moreover, the team’s system holds significant value due to its straightforward manufacturing process that is ready for immediate application.

Professor Soojin Park stressed: “We used a micro-silicon anode, yet, we have a stable battery. This research brings us closer to a real high-energy-density lithium-ion battery system.”

This study was conducted with the support from the Independent Researcher Program of the National Research Foundation of Korea.

In a new study, published today in Royal Society Open Science, findings show that the first dinosaurs were simply faster and more dynamic than their competitors and why they were able to dominate the Earth for 160 million years.

The researchers compared the limb proportions of a broad array of reptiles from the Triassic, the period of time from 252 to 201 million years ago, when dinosaurs first appeared and rose to prominence. They identified which of these ancient beasts was quadrupedal (four-footed) or bipedal (two-footed), and also looked at their cursoriality index, a measure of their running ability.

They found that, from the beginning, not only were the dinosaurs and their close relatives bipedal and cursorial — which meant they had limbs adapted for running, they also showed a much wider range of running styles than some of their close competitors, called the Pseudosuchia.

The pseudosuchians included the ancestors of modern crocodiles. Some were small insect-eating bipeds, but most were medium-to-large-sized carnivores and herbivores and they were diverse throughout the Triassic. The team found that dinosaurs and their kin, the Avemetatarsalia, maintained a higher range of locomotory modes throughout this period.

MSc Palaeobiology student Amy Shipley led the study. She said: “When the crunch came, 233 million years ago, dinosaurs won out.

“At that time, climates went from wet to dry, and there was severe pressure for food. Somehow the dinosaurs, which had been around in low numbers already for 20 million years, took off and the pseudosuchians did not.

“It’s likely the early dinosaurs were good at water conservation, as many modern reptiles and birds are today. But our evidence shows that their greater adaptability in walking and running played a key part.”

“After the end of the Triassic, when there was a mass extinction, the dinosaurs expanded again,” said Professor Mike Benton. “Most of the pseudosuchians were wiped out by the mass extinction, except for the ancestors of crocodiles, and we found that these surviving dinosaurs expanded their range of locomotion again, taking over many of the empty niches.”

Co-author Dr Armin Elsler explained: “When we looked at evolutionary rates, we found that in fact dinosaurs were not evolving particularly fast.

“This was a surprise because we expected to see fast evolution in avemetatarsalians and slower evolution in pseudosuchians. What this means is that the locomotion style of dinosaurs was advantageous to them, but it was not an engine of intense evolutionary selection. In other words, when crises happened, they were well placed to take advantage of opportunities after the crisis.”

“We always think of dinosaurs as huge and lumbering,” says Dr Tom Stubbs, another collaborator. “This reminds us that actually the dinosaurs started as nippy little insect-eaters.

“The first dinosaurs were only a metre long, up high on their legs, and bipedal. Their leg posture meant they could move fast and catch their prey while escaping larger predators.”

Co-author Dr Suresh Singh concluded: “And of course, their diversity of posture and focus on fast running meant that dinosaurs could diversify when they had the chance.

“After the end-Triassic mass extinction, we get truly huge dinosaurs, over ten metres long, some with armour, many quadrupedal, but many still bipedal like their ancestors. The diversity of their posture and gait meant they were immensely adaptable, and this ensured strong success on Earth for so long.”

For biofuels to compete with petroleum, biorefinery operations must be designed to better utilize lignin. Lignin is one of the main components of plant cell walls. It provides plants with greater structural integrity and resiliency from microbial attacks. However, these natural properties of lignin also make it difficult to extract and utilize from the plant matter, also known as biomass.

“Lignin utilization is the gateway to making what you want out of biomass in the most economical and environmentally friendly way possible,” said UC Riverside Associate Research Professor Charles Cai. “Designing a process that can better utilize both the lignin and sugars found in biomass is one of the most exciting technical challenges in this field.”

To overcome the lignin hurdle, Cai invented CELF, which stands for co-solvent enhanced lignocellulosic fractionation. It is an innovative biomass pretreatment technology.

“CELF uses tetrahydrofuran or THF to supplement water and dilute acid during biomass pretreatment. It improves overall efficiency and adds lignin extraction capabilities,” Cai said. “Best of all, THF itself can be made from biomass sugars.”

A landmark Energy & Environmental Science paper details the degree to which a CELF biorefinery offers economic and environmental benefits over both petroleum-based fuels and earlier biofuel production methods.

The paper is a collaboration between Cai’s research team at UCR, the Center for Bioenergy Innovation managed by Oak Ridge National Laboratories, and the National Renewable Energy Laboratory, with funding provided by the U.S. Department of Energy’s Office of Science. In it, the researchers consider two main variables: what kind of biomass is most ideal and what to do with the lignin once it’s been extracted.

First-generation biofuel operations use food crops like corn, soy, and sugarcane as raw materials, or feedstocks. Because these feedstocks divert land and water away from food production, using themfor biofuels is not ideal.

Second-generation operations use non-edible plant biomass as feedstocks. An example of biomass feedstocks includes wood residues from milling operations, sugarcane bagasse, or corn stover, all of which are abundant low-cost byproducts of forestry and agricultural operations.

According to the Department of Energy, up to a billion tons per year of biomass could be made available for the manufacture of biofuels and bioproducts in the US alone, capable of displacing 30% of our petroleum consumption while also creating new domestic jobs.

Because a CELF biorefinery can more fully utilize plant matter than earlier second-generation methods, the researchers found that a heavier, denser feedstock like hardwood poplar is preferable over less carbon-dense corn stover for yielding greater economic and environmental benefits.

Using poplar in a CELF biorefinery, the researchers demonstrate that sustainable aviation fuel could be made at a break-even price as low as $3.15 per gallon of gasoline equivalent. The current average cost for a gallon of jet fuel in the U.S. is $5.96.

The U.S. government issues credits for biofuel production in the form of renewable identification number credits, a subsidy meant to bolster domestic biofuel production. The tier of these credits issued for second-generation biofuels, the D3 tier, is typically traded at $1 per gallon or higher. At this price per credit, the paper demonstrates that one can expect a rate of return of over 20% from the operation.

“Spending a little more for a more carbon-rich feedstock like poplar still yields more economic benefits than a cheaper feedstock like corn stover, because you can make more fuel and chemicals from it,” Cai said.

The paper also illustrates how lignin utilization can positively contribute to overall biorefinery economics while keeping the carbon footprint as low as possible. In older biorefinery models, where biomass is cooked in water and acid, the lignin is mostly unusable for more than its heating value.

“The older models would elect to burn the lignin to supplement heat and energy for these biorefineries because they could mostly only leverage the sugars in the biomass — a costly proposition that leaves a lot of value off the table,” said Cai.

In addition to better lignin utilization, the CELF biorefinery model also proposes to produce renewable chemicals. These chemicals could be used as building blocks for bioplastics and food and drink flavoring compounds. These chemicals take up some of the carbon in the plant biomass that would not get released back into the atmosphere as CO₂.

“Adding THF helps reduce the energy cost of pretreatment and helps isolate lignin, so you wouldn’t have to burn it anymore. On top of that, we can make renewable chemicals that help us achieve a near-zero global warming potential,” Cai said. “I think this moves the needle from Gen 2 biofuels to Gen 2+.”

In light of the team’s recent successes, the Department of Energy’s Bioenergy Technology Office has awarded the researchers a $2 million grant to build a small-scale CELF pilot plant at UCR. Cai hopes that demonstrating the pilot plant will lead to larger-scale investment in the technology, as harnessing energy from fossil fuels adds to global warming and hurts the planet.

“I began this work more than a decade ago because I wanted to make an impact. I wanted to find a viable alternative to fossil fuels and my colleagues and I have done that,” Cai said. “Using CELF, we have shown it is possible to create cost-effective fuels from biomass and lignin and help curb our contribution of carbon emissions into the atmosphere.”