The study, published on December 10 in Aging, analyzed how much theobromine was present in participants’ blood and compared those levels with biological aging markers measured in blood samples.

What Biological Age Reveals

Biological age reflects how well a person’s body is functioning, rather than the number of years they have lived. This measure is based on DNA methylation, a collection of tiny chemical tags on DNA that shift as we grow older.

The research team examined data from two European groups, including 509 people from TwinsUK and 1,160 from KORA. Individuals with higher amounts of theobromine in their bloodstream tended to have a biological age that appeared younger than their chronological age.

Professor Jordana Bell, senior author and Professor in Epigenomics at King’s College London, said: “Our study finds links between a key component of dark chocolate and staying younger for longer. While we’re not saying that people should eat more dark chocolate, this research can help us understand how everyday foods may hold clues to healthier, longer lives.”

Theobromine Stands Out Among Cocoa Compounds

The team also investigated whether other cocoa or coffee metabolites showed similar patterns. Their results suggested that theobromine was the only compound with this specific association.

To estimate biological age, the researchers used two approaches. One method examined DNA changes that reflect the pace of aging. The second measured telomere length, which refers to protective structures at the ends of chromosomes. Telomeres naturally shorten with age and are linked to age-related health risks.

How Plant Molecules Influence Aging

Many plant compounds in foods can influence how genes operate by turning them on or off. These compounds, known as alkaloids, can interact with cellular systems that regulate gene activity and contribute to long-term health.

Theobromine is one such alkaloid. Although it is widely known for being toxic to dogs, it has been connected to possible benefits in humans, including a reduced risk of heart disease. Despite this, it has received relatively limited scientific attention until now.

Next Questions for Aging Research

Dr. Ramy Saad, lead researcher at King’s College London and also affiliated with University College London as a doctor in Clinical Genetics, said: “This is a very exciting finding, and the next important questions are what is behind this association and how can we explore the interactions between dietary metabolites and our epigenome further? This approach could lead us to important discoveries towards aging, and beyond, in common and rare diseases.”

The team, which includes Professor Ana Rodriguez-Mateos, a Professor of Human Nutrition at King’s College London, is now examining whether theobromine’s effect occurs on its own or if it works alongside other well-known components of dark chocolate such as polyphenols, which are recognized for their positive effects on health.

Why More Chocolate Is Not a Shortcut

Dr. Ricardo Costeira, a Postdoctoral Research Associate at King’s College London, said: “This study identifies another molecular mechanism through which naturally occurring compounds in cocoa may support health. While more research is needed, the findings from this study highlight the value of population-level analyses in aging and genetics.”

Although the findings are encouraging, the researchers caution that increasing dark chocolate consumption is not automatically beneficial. Chocolate also contains sugar, fat and other ingredients, and more work is needed to fully understand how theobromine interacts with the body and how it may influence aging.

This achievement came from a project led by Oxford researchers using the SNO+ detector, which sits two kilometers underground at SNOLAB in Sudbury, Canada. SNOLAB operates within an active mine and provides the shielding needed to block cosmic rays and background radiation that would otherwise overwhelm the delicate neutrino measurements.

Capturing a Rare Two-Part Flash From Carbon-13

The research team focused on detecting moments when a high-energy neutrino hits a carbon-13 nucleus and converts it into nitrogen-13, a radioactive form of nitrogen that decays roughly ten minutes later. To spot these events, they relied on a ‘delayed coincidence’ technique that searches for two related bursts of light: the first from the neutrino striking the carbon-13 nucleus and the second from the decay of nitrogen-13 several minutes afterward. This paired signal makes it possible to confidently distinguish true neutrino events from background noise.

Over a span of 231 days, from May 4, 2022, to June 29, 2023, the detector recorded 5.6 such events. This matches expectations, which predicted that 4.7 events would occur due to solar neutrinos during this period.

A New Window Into How the Universe Works

Neutrinos behave in unusual ways and are key to understanding how stars operate, how nuclear fusion unfolds, and how the universe evolves. The researchers say this new measurement opens opportunities for future studies of other low-energy neutrino interactions.

Lead author Gulliver Milton, a PhD student in the University of Oxford’s Department of Physics, said: “Capturing this interaction is an extraordinary achievement. Despite the rarity of the carbon isotope, we were able to observe its interaction with neutrinos, which were born in the Sun’s core and traveled vast distances to reach our detector.”

Co-author Professor Steven Biller (Department of Physics, University of Oxford) added: “Solar neutrinos themselves have been an intriguing subject of study for many years, and the measurements of these by our predecessor experiment, SNO, led to the 2015 Nobel Prize in physics. It is remarkable that our understanding of neutrinos from the Sun has advanced so much that we can now use them for the first time as a ‘test beam’ to study other kinds of rare atomic reactions!”

Building on the SNO Legacy and Advancing Neutrino Research

SNO+ is a successor to the earlier SNO experiment, which demonstrated that neutrinos switch between three forms known as electron, muon, and tau neutrinos as they travel from the Sun to Earth. According to SNOLAB staff scientist Dr. Christine Kraus, SNO’s original findings, led by Arthur B. McDonald, resolved the long-standing solar neutrino problem and contributed to the 2015 Nobel Prize in Physics. These results paved the way for deeper investigations into how neutrinos behave and their significance in the universe.

“This discovery uses the natural abundance of carbon-13 within the experiment’s liquid scintillator to measure a specific, rare interaction,” Kraus said. “To our knowledge, these results represent the lowest energy observation of neutrino interactions on carbon-13 nuclei to date and provides the first direct cross-section measurement for this specific nuclear reaction to the ground state of the resulting nitrogen-13 nucleus.”

The findings, published December 10 in the journal PeerJ, come from a partnership between scientists at the Smithsonian’s National Museum of Natural History and Qatar Museums. The team also identified a previously unknown species of ancient sea cow that was much smaller than modern dugongs.

“We discovered a distant relative of dugongs in rocks less than 10 miles away from a bay with seagrass meadows that make up their prime habitat today,” said Nicholas Pyenson, curator of fossil marine mammals at the National Museum of Natural History and a lead author of the study. “This part of the world has been prime sea cow habitat for the past 21 million years — it’s just that the sea cow role has been occupied by different species over time.”

Modern Dugong Biology and Behavior

Dugongs (Dugong dugon) have a stout body and a downward-facing snout lined with bristles that help them sense food, giving them a broad resemblance to manatees. Their tails distinguish them from their relatives. Manatees have a rounded, paddle-shaped tail while dugongs have a dolphin-like tail with flukes (however, dugongs and manatees are more closely related to elephants than they are to dolphins, whales and porpoises).

These herbivores occupy shallow coastal habitats across a wide range that includes western Africa, the Indo-Pacific, and northern Australia. The largest single herd of dugongs occurs in the Arabian Gulf, where their constant grazing stirs up sediment and releases nutrients that benefit surrounding marine ecosystems.

A Long Fossil History and Growing Modern Threats

Fossil evidence shows that sea cow ancestors have fed on aquatic plants for roughly 50 million years. Despite this long history, dugongs in the Gulf now face significant challenges. They are sometimes caught accidentally by local fishers, and development along the coast affects the waters where they feed. Rising temperatures and increasing salinity place further pressures on the seagrass meadows that dugongs depend on.

Ferhan Sakal, head of excavation and site management at Qatar Museums and a coauthor of the study, noted that crucial information about past seagrass environments is preserved in the region’s rock record.

“If we can learn from past records how the seagrass communities survived climate stress or other major disturbances like sea-level changes and salinity shifts, we might set goals for a better future of the Arabian Gulf,” he said.

Researchers rely heavily on fossilized bones to understand these environments, since the soft blades of seagrass rarely leave impressions in the geologic record.

Exploring the Al Maszhabiya Fossil Site

One of the most significant sources of these fossils is Al Maszhabiya [AL mahz-HA-bee-yah], a site in southwestern Qatar. Geologists first encountered the site in the 1970s while conducting mining and petroleum surveys and believed they had found reptile bones. When paleontologists revisited the area in the early 2000s, they recognized the bones as belonging to ancient sea cows.

“The area was called ‘dugong cemetery’ among the members of our authority,” Sakal said. “But at the time, we had no idea just how rich and vast the bonebed actually was.”

After obtaining the required permits in 2023, Pyenson, Sakal, and their team surveyed the site. Surrounding rock layers suggest that the fossils date to the Early Miocene, approximately 21 million years ago. The area was once a shallow sea inhabited by sharks, barracuda-like fish, prehistoric dolphins, and sea turtles.

The World’s Densest Sea Cow Bonebed

The team documented sea cow remains at more than 170 separate locations across the site. Pyenson described Al Maszhabiya as the richest fossil sea cow assemblage known. He compared it to Cerro Ballena in Chile’s Atacama Desert, where he and other researchers had uncovered a large collection of whale fossils.

Although the bones share similarities with those of modern dugongs, they also show differences. The ancient animals still had hind limb bones, which living dugongs and manatees lost during their evolution. The prehistoric species also had a straighter snout and smaller tusks.

Naming a New Species: Salwasiren qatarensis

The team formally designated the Al Maszhabiya sea cows as a new species, Salwasiren qatarensis. The genus name refers to the Bay of Salwa, a nearby section of the Gulf where dugongs live today. Although the Bay of Salwa touches the waters of several countries, the species name “qatarensis” honors Qatar, where the fossils were discovered.

“It seemed only fitting to use the country’s name for the species as it clearly points to where the fossils were discovered,” Sakal said.

Based on their estimates, the researchers believe Salwasiren weighed around 250 pounds, similar to the weight of an adult panda or a heavyweight boxer. Even at that size, it was relatively small compared with some dugongs living today, which can weigh nearly eight times more.

Ancient Seagrass Meadows and the Role of Sea Cows

The fossils provide evidence that abundant seagrass beds existed in the region more than 20 million years ago, during a period when the Gulf supported high marine biodiversity. Sea cows would have helped maintain these underwater meadows by feeding and disturbing the sediment.

“The density of the Al Maszhabiya bonebed gives us a big clue that Salwasiren played the role of a seagrass ecosystem engineer in the Early Miocene the way that dugongs do today,” Pyenson said. “There’s been a full replacement of the evolutionary actors but not their ecological roles.”

Pyenson also noted that sea cow fossils often appear in mixed species groups, making it likely that further research at the site could uncover additional dugong relatives.

Preserving Qatar’s Fossil Heritage

Sakal hopes continued collaboration between Qatar Museums and the Smithsonian will lead to further discoveries at Al Maszhabiya and other nearby locations. Protecting the site is a top priority, and the team plans to nominate it for recognition as a UNESCO World Heritage site.

“The most important part of our collaboration is ensuring that we provide the best possible protection and management for these sites, so we can preserve them for future generations,” Sakal said.

“Dugongs are an integral part of our heritage, not only as a living presence in our waters today, but also in the archaeological record that connects us to generations past,” said Faisal Al Naimi, coauthor and director of the Archaeology Department at Qatar Museums. “The findings at Al Maszhabiya remind us that this heritage is not confined to memory or tradition alone, but extends deep into geologic time, reinforcing the timeless relationship between our people and the natural world. In preserving and studying these remarkable creatures, we are also safeguarding a narrative that speaks to our nation’s identity, resilience and enduring connection to the sea.”

Digital Access and Continued Research

To make their data widely available, Pyenson and Sakal worked with the Smithsonian’s Digitization Program Office to create digital scans of several fossil sites and of the fossil skull, vertebrae, tooth, and other skeletal parts of the newly described species. These 3D models can be explored through the open-source Smithsonian Voyager platform, which includes interactive educational materials and a virtual tour of the excavation.

The study’s authors also include researchers from the Smithsonian’s Digitization Program Office, the Stone Ridge School of the Sacred Heart, Texas A&M University at Galveston, Texas A&M University College Station, and the Natural History Museum of Los Angeles County.

This work was supported by a collaborative agreement between the Smithsonian Institution and Qatar Museums and received additional funding from the National Museum of Natural History and the Qatar National Research Fund.