The conventional understanding of evolution is that genes encoding a beneficial function are the most frequently transmitted, which ensures that the fittest organisms — the ones that have traits most favorable for their environment — survive. Less known is the fact that there exist parasitic gene elements within an organism that are doing just the opposite.

“The way one could think of it is that the genome is like a society,” explains Zanders. “Within that society, there are individuals who derive their living from doing good things and making valuable contributions. But there are others who don’t contribute in beneficial ways and are actually harmful to society,” explains Zanders.

The Zanders Lab studies parasitic genes in Schizosaccharomyces pombe, a species of fission yeast sometimes found in the popular fermented tea drink kombucha. Because of its simplicity and fast generation times, S. pombe is a highly tractable system for studying parasitic gene elements, particularly a class known as meiotic drivers. Instead of conferring a benefit to their host, meiotic drivers confer distinct disadvantages to their host, in order to bias their own perpetuation. One method of doing so, employed by the wtf meiotic drive genes, is through selective poisoning of cells in meiosis.

Meiosis is the process of cell division through which sexually-reproducing organisms form gametes — such as egg and sperm cells in humans, or spores in yeast — to propagate the next generation. Normally, this process results in gametes that inherit one of two copies of each chromosome carried by the parent cell, and each copy is transmitted to gametes at an equal rate. Meiotic drivers, however, short-circuit this law of Mendelian segregation.

“Usually all the alleles — or variants of a particular gene — get a fair chance, and natural selection can pick the best ones,” explains Zanders. “But alleles that are meiotic drivers select themselves even if they’re not the best option. And they’re never the best option.”

In a paper published online August 13, 2020, in eLife, members of the Zanders lab explain how it could be possible that meiotic drivers persist in the population, even as they kill off many would-be hosts. It turns out that S. pombe can employ variants of other genes to help suppress the negative effect of meiotic drivers, albeit at a cost to fitness.

Usually, to propagate laboratory strains of S. pombe, they are inbred. “When you outcross two isolates that are almost identical, they produce almost no progeny,” explains first author María Angélica Bravo Núñez, PhD, who was involved in the identification of the wtf genes, and who did this work in the Zanders Lab as a predoctoral researcher at the Graduate School of the Stowers Institute. “This suggested there might be some type of competition at play.” Bravo Núñez and colleagues used this reasoning to seek out genes that could be in conflict in similar, but non-identical, genomes of S. pombe. The central experimental design of the current work involved outcrossing of S. pombe isolates that are over 99% identical.

“Outcrossing can have many advantages,” says Bravo Núñez, such as providing a normal allele of a gene to rescue the effect of a mutant allele. “But the meiotic drive genes that we study actually exert their deleterious effect in the heterozygous scenario, where the alleles of a gene are not the same.”

One illuminating experiment they did was to compare outcomes of inbred and outcrossed S. pombe isolates in a genetic background containing a mutation in rec12, which is a gene that promotes proper chromosome segregation.

“Rec12 usually promotes fertility,” says Bravo Núñez. “When we removed rec12 function, the numbers of viable spores decreased, but only in the inbred scenario. The relative numbers of viable spores didn’t really decrease in the outcrossed scenario.” Moreover, of the viable spores, they found that survival was biased towards atypical gamete products, resulting from mis-segregation and unequal crossover of paired chromosomes. In other words, surviving spores contained the maximal number of meiotic drivers, sometimes due to inheriting an extra chromosome. This finding was surprising because extra chromosomes are usually considered very harmful. In humans, for instance, extra or missing chromosomes can lead to inviable gametes, severe birth defects, or infertility.

“When you have heterozygosity of wtf genes, making a mutation in the rec12 gene is relatively good for the organism, because that’s just what has to happen in order to not kill all the progeny,” says Bravo Núñez.

“We think of rec12 as this gene that’s really important for fertility,” explains Zanders. “But the situation is totally different when there is outcrossing. Having rec12 doesn’t actually help at all, because the yeast is better off making gametes that do not result from proper chromosome segregation. This is just one example of the power of meiotic drivers to change the landscape of meiosis. Fundamentally, what is ‘good’ for the organism has changed.”

“Having that extra chromosome is not good, and the yeast colonies look unusual, small, and irregular. But after they continue to grow for a while, the cells lose that extra chromosome and then they can thrive as haploids. So, this step is actually just temporary,” says Bravo Núñez.

Precisely because meiotic drivers exert their influence in a heterozygous scenario, they are easy to miss. “There are many flavors of meiotic drive. Some forms of meiotic drive are hard to measure experimentally because the bias is so subtle,” says Zanders. “We’re not the first to study meiotic drivers in depth. We just have a better model system now, so we can make more progress faster.”

“Drive systems tend to be repetitive, and you can usually find them in various copies in genomes,” says Bravo Núñez. “They have, in many cases, already been found in other systems, such as fungi, mice, and fruit flies, but are not yet fully characterized.” The study of meiotic drivers in S. pombe “will hopefully guide future research to understand other drive systems.”

“Humans certainly have meiotic drive genes. Whether or not they have meiotic drive genes of the gamete-killing type is unclear,” says Zanders. “Meiotic drive has likely affected the evolution of human centromeres, which are regions of chromosomes that are very important for proper chromosome segregation. Certain chromosome fusions exhibit meiotic drive in humans, as do sequences that are involved in DNA recombination. We’re going to continue focusing on these and other parasitic gene elements, their strategies, and their effects.”

Other coauthors of this work include Ibrahim M. Sabbarini and Lauren E. Eide from the Stowers Institute and Robert L. Unckless, PhD, from the University of Kansas.

This work was funded in part by the Stowers Institute for Medical Research, March of Dimes Foundation Basil O’Connor Starter Scholar Research Award (5-FY18-58 to SEZ), Searle Scholar Award (to SEZ), and National Institutes of Health (National Institute of General Medical Sciences awards R00GM114436 and DP2GM132936 to SEZ and National Cancer Institute award F99CA234523 to MABN). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

A new study found that if EVs replaced 25% of combustion-engine cars currently on the road, the United States would save approximately $17 billion annually by avoiding damages from climate change and air pollution. In more aggressive scenarios — replacing 75% of cars with EVs and increasing renewable energy generation — savings could reach as much as $70 billion annually.

“Vehicle electrification in the United States could prevent hundreds to thousands of premature deaths annually while reducing carbon emissions by hundreds of millions of tons,” said Daniel Peters, who led the study. “This highlights the potential of co-beneficial solutions to climate change that not only curb greenhouse gas emissions but also reduce the health burden of harmful air pollution.”

“From an engineering and technological standpoint, people have been developing solutions to climate change for years,” added Northwestern’s Daniel Horton, senior author of the study. “But we need to rigorously assess these solutions. This study presents a nuanced look at EVs and energy generation and found that EV adoption not only reduces greenhouse gases but saves lives.”

The study was published online last week (August 13) in the journal GeoHealth.

During this research, Peters was an undergraduate researcher in Horton’s laboratory at Northwestern. He now works for the Environmental Defense Fund. Horton is an assistant professor of Earth and planetary sciences in Northwestern’s Weinberg College of Arts and Sciences.

To conduct the study, Horton, Peters and their team looked at vehicle fleet and emissions data from 2014. If 25% of U.S. drivers adopted EVs in 2014 — and the power required to charge their batteries came from 2014’s energy generation infrastructure — then 250 million tons of carbon dioxide (CO₂) emissions would have been mitigated. Although the impact of carbon emissions on the climate is well documented, combustion engines also produce other harmful pollutants, such as particulate matter and the precursors to ground-level ozone. Such pollutants can trigger a variety of health problems, including asthma, emphysema, chronic bronchitis and ultimately premature death.

After leaving tailpipes and smokestacks, pollutants interact with their environment, including background chemistry and meteorology.

“A good example is to look at nitrogen oxides (NO_x), a group of chemicals produced by fossil-fuel combustion,” Peters explained. “NO_x itself is damaging to respiratory health, but when it’s exposed to sunlight and volatile organic compounds in the atmosphere, ozone and particulate matter can form.”

To account for these interactions, the researchers used a chemistry-climate model developed at the Geophysical Fluid Dynamics Laboratory. Jordan Schnell, a postdoctoral fellow in Horton’s lab, performed the model experiments that simultaneously simulate the atmosphere’s weather and chemistry, including how emissions from combustion engines and power generation sources interact with each other and other emissions sources in their environments.

With this model, the researchers simulated air pollutant changes across the lower 48 states, based on different levels of EV adoption and renewable energy generation. Then, they combined this information with publicly available county health data from the U.S. Environmental Protection Agency (EPA). This combination enabled the research team to assess health consequences from the air quality changes caused by each electrification scenario.

The research team assigned dollar values to the avoided climate and health damages that could be brought about by EV adoption by applying the social cost of carbon and value of statistical life metrics to their emission change results. These commonly used policy tools attach a price tag to long-term health, environmental and agricultural damages.

“The social cost of carbon and value of statistical life are much-studied and much-debated metrics,” Horton said. “But they are regularly used to make policy decisions. It helps put a tangible value on the consequences of emitting largely intangible gases into the public sphere that is our shared atmosphere.”

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Materials provided by Northwestern University. Original written by Amanda Morris. Note: Content may be edited for style and length.

The research team, comprising scientists from Germany, Spain, China and the U.S., rigorously analysed more than ten years of data from the US space administration NASA’s Fermi gamma-ray space telescope, looking at a so-called micro quasar. The system catalogued as SS 433 is located some 15,000 lightyears away in the Milky Way and consists of a giant star with about 30 times the mass of our sun and a black hole with about 10 to 20 solar masses. The two objects are orbiting each other with a period of 13 days, while the black hole sucks matter from the giant star.

“This material accumulates in an accretion disc before falling into the black hole, like water in the whirl above the drain of a bath tub,” explains Li. “However, a part of that matter does not fall down the drain but shoots out at high speed in two narrow jets in opposite directions above and below the rotating accretion disk.” This setting is known from active galaxies called quasars with monstrous black holes with millions of solar masses at their centres that shoot jets tens of thousands of lightyears into the cosmos. As SS 433 looks like a scaled-down version of these quasars, it has been dubbed a micro quasar.

The high-speed particles and the ultra-strong magnetic fields in the jet produce X-rays and gamma rays. “The accretion disc does not lie exactly in the plane of the orbit of the two objects. It precesses, or sways, like a spinning top that has been set up slanted on a table,” says Torres. “As a consequence, the two jets spiral into the surrounding space, rather than just forming a straight line.”

The precession of the black hole’s jets has a period of about 162 days. Meticulous analysis revealed a gamma-ray signal with the same period from a position located relatively far from the micro quasar’s jets, which has been labelled as Fermi J1913+0515 by the scientists. It is located at the position of an unremarkable gas enhancement. The consistent periods indicate the gas cloud’s emission is powered by the micro quasar.

“Finding such an unambiguous connection via timing, about 100 light years away from the micro quasar, not even along the direction of the jets is as unexpected as amazing,” says Li. “But how the black hole can power the gas cloud’s heartbeat is unclear to us.” Direct periodic illumination by the jet seems unlikely. An alternative that the team explored is based on the impact of fast protons (the nuclei of hydrogen atoms) produced at the ends of the jets or near the black hole, and injected into the cloud, where these subatomic particles hit the gas and produce gamma rays. Protons could also be part of an outflow of fast particles from the edge of the accretion disc. Whenever this outflow strikes the gas cloud, it lights up in gamma rays, which would explain its strange heartbeat. “Energetically, the outflow from the disc could be as powerful as that of the jets and is believed to precess in solidarity with the rest of the system,” explains Torres.

Further observations as well as theoretical work are required to fully explain the strange gamma-ray heartbeat of this unique system beyond this initial discovery. “SS 433 continues to amaze observers at all frequencies and theoreticians alike,” emphasises Li. “And it is certain to provide a testbed for our ideas on cosmic-ray production and propagation near micro quasars for years to come.”

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Materials provided by Deutsches Elektronen-Synchrotron DESY. Note: Content may be edited for style and length.